MicroRNA-181b和microRNA-130a在冠状动脉粥样硬化性心脏病患者血浆中的表达及临床意义

Expressions of miR-181b and miR-130a in plasma of patients with coronary heart disease and clinical significance

摘要:

目的探讨microRNA-181b（miR-181b）和microRNA-130a（miR-130a）在冠状动脉粥样硬化性心脏病（简称冠心病）患者血浆中的表达及临床意义。方法选取106 例冠心病患者和40 例健康者作为对照组，收集临床资料，行冠状动脉造影检查及Gensini评分，利用实时荧光定量聚合酶链反应（qRT-PCR）检测冠心病患者和对照组血浆中miR-181b 和miR-130a 的表达，利用受试者工作特征曲线（ROC 曲线）对血浆中miR-181b 和miR-130a 的相对表达量在评估冠心病患者病程进展中的价值进行分析。结果冠心病患者血浆中miR-181b 和miR-130a 相对表达量与对照组比较，差异有统计学意义（P <0.05），冠心病患者血浆中miR-181b 相对表达量低于对照组，而miR-130a 相对表达量则高于对照组，冠心病患者中，中重度组患者血浆miR-181b 相对表达量低于轻度组，而miR-130a 相对表达量则高于轻度组；Pearson 相关分析显示，冠心病患者血浆中miR-181b 相对表达量均与Gensini 积分、脂蛋白a（Lp-a）、高敏C 反应蛋白（hs-CRP）呈负相关（r =-0.504、-0.382 和-0.415，p <0.05），血浆中miR-130a 相对表达量均与Gensini 积分、Lpa 和hs-CRP 呈正相关（r =0.586、0.335 和0.394，p <0.05）；ROC 曲线分析显示，冠心病患者血浆中miR-181b 相对表达量在评估患者病程进展时，曲线下面积0.871（95%CI：0.807，0.936），敏感性75.0%，特异性82.3%，血浆中miR-130a 相对表达量在评估患者病程进展时，曲线下面积0.931（95%CI：0.887，0.976），敏感性93.2%，特异性81.2%。结论冠心病患者血浆中miR-181b 表达水平降低，而miR-130a 表达水平升高，且均与患者病情严重程度有关，可作为评估患者病情进展的指标。

Abstract:

Objective To investigate the expressions of miR-181b and miR-130a in the plasma of patients with coronary heart disease and their clinical significance. Methods In this study 106 cases of patients with coronary heart disease and 40 cases of healthy controls were selected. The clinical data were collected, and the coronary angiograms and Gensini score were taken. The expressions of miR-181b and miR-130a in plasma were detected using RT-PCR. The values of the relative expression levels of miR-181b and miR-130a in assessing disease progression in the patients with coronary heart disease were analyzed by receiver operating characteristic curve (ROC curve). Results The relative expression level of miR-181b in the plasma of the patients with coronary heart disease was lower than that of the control group, while the relative expression level of miR-130a was higher than that of the control group (P < 0.05), Among the patients with coronary heart disease, the relative expression level of miR-181b in the plasma of the patients in the severe group was lower than that in the mild group, while the relative expression level of miR-130a was higher than that in the mild group, the differences were statistically significant (P < 0.05).Pearson correlation analysis showed that the relative expression level of miR-181b in the plasma of the patients with coronary heart disease was negatively correlated with Gensini score, Lpa and hs-CRP (R = -0.504, -0.382 and -0.415;P < 0.05); and the relative expression level of miR-130a was positively correlated with Gensini score, Lpa and hs-CRP (R = 0.586, 0.335 and 0.394;P < 0.05). ROC curve analysis showed that using the relative expression level of miR -181b in the plasma of the patients with coronary heart disease for assessment of the disease progression, the area under the curve (AUC) was 0.871 (95% CI: 0.807, 0.936), the sensitivity was 75.0% and the specificity was 82.3%; and for the relative expression level of miR-130a, the AUC was 0.931 (95% CI: 0.887,0.976), the sensitivity was 93.2% and and the specificity was 81.2%, respectively. Conclusions In the plasma of the patients with coronary heart disease, the expression level of miR-181b decreases, and miR-130a increases, and they were related with the severity of the disease. They could be used as the indicators to assess the disease progression.