Abstract:Objective To investigate the effect of work stress on electrocardiogram (ECG) of healthy people. Methods This study included 282 healthy people who had physical examination in our hospital between June 2015 and January 2016. According to the Occupational Stress Scale (OSI) ratings, they were divided into highlevel job stress group (≤ 15 points) and low-level job stress group ( > 15 points). ECG abnormalities were compared between the two groups. Results In the 282 cases of physical examination, ECG abnormalities were found in 57 cases, including ST-T segment changes in 21 cases, Q-Tc interval prolongation in 15 cases, P-R interval prolongation in 12 cases, heart block in 9 cases, sinus arrhythmia in 6 cases, arrhythmia in 6 cases, left axis deviation in 3 cases,atrial fibrillation in 3 cases, premature beats in 3 cases, the other abnormalities in 9 cases. In the 159 cases of the high-level job stress group 45 cases had abnormal ECG (28.30%), while in the 123 cases of the low-level job stress group ECG abnormalities appeared in 12 cases (9.76%). In the high-level job stress group, ECG abnormality rates of the populations with the age ≤40 years, 41-59 years and ≥60 years were 10.00%, 30.00% and 38.46% respectively;in the low-level job stress group, ECG abnormality rates of the populations with the age ≤40 years, 41-59 years and ≥60 years were 7.69%, 5.56% and 20.00% respectively. The abnormal ECG rates of the 41-59 years old crowds and the ≥60 years old crowds were statistically different between the two groups (P < 0.05). Age, type of work and work stress were the independent factors influencing ECG findings of healthy people (P < 0.05). Conclusions Job stress is an independent risk factor for ECG abnormalities mainly manifested by ST-T segment changes and Q-Tc interval prolongation. The higher the stress level, the higer the abnormal ECG rate, and the greater the impact on the elderly population.