干细胞转录因子OCT4、SOX2在食管鳞癌中的表达及与预后的关系

Expressions of OCT4 and SOX2 in esophageal squamous cell carcinoma and their relationships with clinical prognosis

摘要:

探讨干细胞转录因子Sry 相关HMG 盒2（SOX2）、八聚体结合转录因子4（OCT4）在食管鳞癌中的表达变化及与患者临床特征、预后的关系。方法采用免疫组织化学SP 法检测77 例手术后经病理学证实为食管鳞癌的手术标本（食管癌组）和40 例癌旁组织标本（癌旁组织）中SOX2、OCT4 蛋白的表达水平，并分析其与患者临床特征、预后的关系。结果食管癌组织中的SOX2 蛋白、OCT4 蛋白阳性表达率分别为63.64%和77.92%均高于癌旁组织的17.50%和15.00%，且差异具有统计学意义（p <0.05）；食管癌组织中的SOX2 蛋白、Oct4 蛋白在低分化、淋巴结转移患者中呈高表达（p <0.05）；食管癌组织中的SOX2 蛋白阳性表达患者的3 年随访中位生存期24 个月低于阴性患者的30 个月（x2=4.072， p=0.044）；OCT4 蛋白阳性表达患者的中位生存时间21 个月低于阴性患者33 个月（x2=4.880，p =0.037）。结论SOX2、OCT4 蛋白在食管癌组织中高表达，并且与患者的分化程度、转移及预后有关。

Abstract:

To investigate the expressions of stem cell transcription factor Sry related HMG box 2(SOX2) and eight dimer binding transcription factor 4 (OCT4) in esophageal squamous cell carcinoma and their re- lationships with clinical characteristics and prognosis. Methods The expression levels of SOX2 and OCT4 proteins were detected by immunohistochemical SP method in 77 cases of esophageal squamous cell carcinoma (esophageal cancer) tissues and 40 cases of paracancerous tissues (adjacent tissues), and their correlations with the clinical fea-tures and prognosis of the patients were analyzed. Results The positive expression rates of SOX2 protein and OCT4 protein in the esophageal cancer tissues (63.64% and 77.92% respectively) were significantly higher than those in the adjacent tissues (17.50% and 15.00% respectively, p< 0.05). SOX2 protein and OCT4 protein were highly ex-pressed in the esophageal cancer tissues of the patients with poorly-differentiated cancer and lymph node metastasis( p< 0.05). In the patients with positive expression of SOX2 protein in the esophageal carcinoma tissues, the median survival time during the three-year follow-up was 24 months, which was significantly shorter than that (30 months) of the SOX2 negative patients (x2 = 4.072, p= 0.044). The median survival time of the patients with positive OCT4 protein expression (21 months) was significantly shorter than that (33 months) of the OCT4 negative patients (x2 =4.880,p = 0.037). Conclusions SOX2 and OCT4 proteins are highly expressed in esophageal cancer, and correlated with the degree of differentiation, metastasis and prognosis.