Survivin mRNA 和CK19 mRNA 在胃癌患者外周血中的表达及临床意义

Expressions of Survivin mRNA and CK19 mRNA in peripheral blood of patients with gastric cancer and clinical significance

摘要:

目的 探讨Survivin mRNA 和CK19 mRNA 在胃癌患者外周中的表达及其在胃癌诊断及预后方面的价值。方法 利用逆转录聚合酶链反应（RT-PCR）检测89 例胃癌患者及健康对照外周血中SurvivinmRNA 和CK19 mRNA 的表达情况，并利用统计学方法分析血清Survivin mRNA 和CK19 mRNA 在胃癌中的诊断和预后价值。结果 RT-PCR 结果发现胃癌患者外周血中Survivin mRNA 和CK19 mRNA 的表达均高于健康对照组；ROC 曲线显示外周血中Survivin mRNA 和CK19 mRNA 为胃癌有效诊断分子靶点；χ2检验显示外周血中Survivin mRNA 和CK19 mRNA 与胃癌患者的TNM 分期呈正相关；Kaplan-Meier 生存曲线显示Survivin 和CK19 双阳性组的胃癌患者术后5 年生存率低于其他各组。结论 胃癌患者外周血中Survivin mRNA 和CK19 mRNA 表达水平增高，是胃癌早期诊断和判断预后的潜在分子指标。

Abstract:

Objective To explore the expressions of Survivin mRNA and CK19 mRNA in the peripheral blood of the patients with gastric cancer and their clinical significance. Methods Expressions of Survivin mRNA and CK19 mRNA in peripheral blood were analyzed by RT-PCR in 89 patients with gastric cancer. Statistical analysis was conducted to analyze the diagnostic and prognostic value of Survivin mRNA and CK19 mRNA in the peripheral blood of the patients with gastric cancer. Results RT-PCR revealed that the Survivin mRNA and CK19 mRNA were both significantly higher in the peripheral blood of the patients with gastric cancer than in the normal controls.ROC curves revealed that both Survivin mRNA and CK19 mRNA were promising diagnostic biomarkers for gastric cancer. Chi-square test revealed that both Survivin mRNA and CK19 mRNA expressions were positively associated with TNM stage of the patients with gastric cancer. Kaplan-Meier curves revealed that the patients with gastric cancer which were both Survivin-positive and CK19-positive had the lowest 5-year survival rate after surgery, which was statistically significant. Conclusions Survivin mRNA and CK19 mRNA are upregulated in the peripheral blood of the patients with gastric cancer. They are promising biomarkers for early diagnosis and prognostic prediction of the patients with gastric cancer.