脂肪移植治疗DNCB诱导ACD模型小鼠的实验研究

Research on fat transplantation treatment for DNCB-sensitized allergic contact dermatitis of mouse model

摘要:

探究脂肪移植治疗2，4- 二硝基氯苯（DNCB）诱导变应性接触性皮炎（allergic contact dermatitis，ACD）模型小鼠的效果及其可能机制。方法采用DNCB 复制ACD小鼠模型，随机分成A（脂肪移植治疗组）、B（假手术治疗组）、C（药物治疗组）、D 组（自然修复组），观察脂肪移植对ACD 小鼠的治疗效果。2 周后处死小鼠，取背部皮损处全层皮肤组织，苏木精- 伊红染色法染色观察各组小鼠皮肤组织病理改变；实时荧光定量聚合酶链反应（qRT-PCR）检测皮损中白介素10（IL-10）、白介素17（IL-17）、干扰素-γ（IFN-γ）的表达。结果A、C 组可以观察到表皮厚度恢复正常，角质层变薄；B、D 组无明显改变。RT-PCR 检测发现A、C组皮肤组织中IL-10 和IL-17 mRNA 水平低于D 组，IFN-γ mRNA 较D 组升高（p <0.05），而B 与D 组比较差异无统计学意义（ p>0.05）。结论脂肪移植可降低皮损中IL-10 和IL-17 的表达，促进IFN-γ产生，减轻皮损炎症。

Abstract:

To explore whether fat transplantation can improve the symptoms of the mouse model of allergic contact dermatitis (ACD) induced by dinitrochlorobenzene (DNCB) as well as its possible mecha-nism. Methods ACD mouse models were randomly divided into group A (fat transplantation group), group B(pseudo surgery group), group C (medication group) and group D (spontaneously-recovered model group). The HE-stained skin tissues of each group were observed under light microscope. The expression levels of IL-10, IL-17 and IFN-γmRNAs in the skin lesions were detected by qRT-PCR. Results In the groups A and C,the horny layers became thinner, and the thickness of epidermis recovered to normal while there were no overt changes in the groups B and D. The expression levels of IL-10 and IL-17 mRNAs in the skin tissues of the groups A and C were clearly lower than those of the group D, the level of IFN-γmRNA was higher than that of the group D (p < 0.05); however, there was no significant changes in the IL-10, IL-17 or IFN-γ mRNA level between the groups B and D. Conclusions Fat transplantation can decrease the expression levels of IL-10 and IL-17 in skin lesions, promote the production of IFN-γ, reduce the symptoms of skin lesions and promote the recovery of skin lesions in the mouse model.