膀胱尿路上皮癌中PTEN表达与其基因甲基化的关系

Correlation between expression of PTEN and its promoter methylation in bladder urothelial cell carcinoma

摘要:

探讨膀胱尿路上皮癌（BUCC）中第10号染色体同源丢失性磷酸酶-张力蛋白基因（ PTEN）表达与其基因启动子甲基化的关系。方法应用Western blot、甲基化特异性聚合酶链反应（MSP），检测41 例BUCC和18 例正常膀胱组织中PTEN蛋白的表达水平及其基因启动子甲基化状态，并分析两者的相关性。结果BUCC 中PTEN 蛋白表达低于正常膀胱组织（p <0.05），而BUCC 中基因启动子甲基化率为53.66%（22/41），高于正常膀胱组织的0.00%（0/18）（ p<0.05）；与甲基化阴性组的BUCC 组织相比，22 例甲基化阳性 组的PTEN 表达水平降低（ p<0.05）。结论BUCC 中PTEN 蛋白的表达水平下调，这与其基因启动子高甲基化密切相关。

Abstract:

To investigate the relationship between expression of phosphate and tension homology deleted on chromsome ten ( PTEN) gene and its promoter methylation in bladder urothelial cell carcinoma (BUCC). Methods The protein expression and promoter methylation of gene were detected in 41 cases of BUCC and 18 normal bladder tissues using Western blot and Methylation Specific PCR (MSP) method respectively, and their correlation was analyzed. Results PTEN protein expression in the BUCC was lower than that in the normal bladder tissues (p < 0.05). And the promoter methylation rate of gene in theBUCC (53.66%, 22/41) was significantly higher than that (0.00%, 0/18) in the normal bladder tissues (p < 0.05). The expression level of PTEN protein in the BUCC of the methylation negative group was obviously lower than that of the methylation positive group ( p< 0.05). Conclusions The low expression of PTEN protein in BUCC is closely related with the high methylation of gene promoter.