岩鹿乳康胶囊对乳腺增生大鼠乳腺组织及雌孕激素受体表达的影响

Effect of Yanlurukang Capsules on breast tissue and expressions of ER and PR in rats with hyperplasia of mammary glands

摘要:

观察岩鹿乳康胶囊对乳腺增生大鼠乳腺组织及雌孕激素受体表达的影响，探讨其治疗乳腺增生的作用机制。方法用肌内注射外源性苯甲酸雌二醇及黄体酮的方法复制大鼠乳腺增生模型，观察各组大鼠乳腺组织变化，用免疫组织化学法检测雌激素受体亚型（ERα、ERβ）和孕激素受体（PR）的表达水平。结果岩鹿乳康胶囊能减少实验大鼠乳腺导管上皮细胞层数和腺泡数，同时降低乳腺组织ERα、PR的表达提高ERβ的表达，抑制乳腺组织增生。结论岩鹿乳康胶囊对大鼠乳腺增生具有抑制作用，其治疗机制可能与上调ERβ，下调ERα、PR 的表达有关。

Abstract:

To observe the effect of Yanlurukang Capsules on the breast tissue and expressions of estrogen receptor (ER) and progesterone receptor (PR) in rats with hyperplasia of mammary glands (HMG), and to discuss the mechanism. Methods Breast hyperplasia rat model was established by intramuscular injection of estradiol benzoate and progesterone. The changes of breast tissue were observed in rats of each group. The expressions of ERα, ERβand PR in breast tissues were detected using immunohistochemical staining. Results Yanlurukang Capsules significantly reduced the layers of mammary ductal epithelial cells and the number of acini in the experimental rats, down-regulated the expressions of ERα and PR, and enhanced the expression of ERβ. Conclusions Yanlurukang Capsules can inhibit HMG in rats, the mechanism may be related to up-regulation of ERβexpression and down-regulation of ERαand PR expressions.