后膜骨架蛋白1a 在小鼠脑缺血再灌注损伤中的作用

Study on effect of Homer 1a on cerebral ischemia-reperfusion injury in mice

摘要:

目的 探讨后膜骨架蛋白1a（Homer 1a）在小鼠脑缺血再灌注损伤中的作用。方法 以Homer1a 基因敲除小鼠为研究对象，复制脑缺血再灌注小鼠模型，根据行为学评分标准评价小鼠脑缺血再灌注的损伤程度。运用TTC 染色法观察计算小鼠脑梗死体积。Tunel 法检测脑缺血再灌注小鼠脑组织中细胞凋亡情况。GFAP 法检测星形胶质细胞的增殖情况。Western blot 检测小鼠脑组织中IL-1β、TNF-α、IL-6 水平。结果 实验组小鼠行为学评分高于脑缺血组，说明Homer 1a 基因敲除小鼠脑缺血再灌注损伤较脑缺血组更为严重。实验组小鼠脑梗死体积与脑缺血组比较，差异有统计学意义（P <0.05），实验组增多。实验组小鼠脑组织细胞凋亡高于脑缺血组（P <0.05）。实验组和脑缺血组小鼠脑组织中星形胶质细胞数都高于正常组（P <0.05），而实验组小鼠脑组织中星型胶质细胞数量低于脑缺血组。实验组小鼠脑组织中IL-1β、TNF-α、IL-6 表达水平均高于脑缺血组（P <0.05）。结论 Homer 1a 基因敲除小鼠可以加重脑缺血再灌注后的损伤。Homer 1a 可以减弱脑缺血再灌注损伤。

Abstract:

Objective To investigate the effect of Homer 1a on cerebral ischemia-reperfusion injury in mice. Methods Homer 1a knockout mice were used as the research objects to construct the model of cerebral ischemia-reperfusion in mice. The degree of cerebral ischemia-reperfusion injury in mice was evaluated based on the behavioral score. TTC staining was used to observe and calculate the volume of cerebral infarction. Apoptosis of the brain tissues of the mice was detected by Tunel. The proliferation of astrocytes was detected by GFAP method. The levels of IL-1β, TNF-α and IL-6 in the mouse brain were detected by Western blot. Results The behavioral score of the experimental group was significantly higher than that of the cerebral ischemia group, indicating that cerebral ischemia-reperfusion injury of the Homer 1a gene knockout mice were more severe than that of the cerebral ischemia group. The cerebral infarction volume in the experimental group was significantly larger than that in the cerebral ischemia group (P < 0.05). The apoptosis rate of brain cells in the experimental group was significantly higher than that in the cerebral ischemia group (P < 0.05). The number of brain astrocytes in the experimental group and the cerebral ischemia group was larger than that in the normal group (P < 0.05), while the number of brain astrocytes in the experimental group was smaller than that in the cerebral ischemia group. The expression levels of IL-6,TNF-α and IL-1β in the brain tissue of the experimental group were higher than those in the cerebral ischemia group (P < 0.05). Conclusions Homer 1a knockout can aggravate the injury after cerebral ischemia and reperfusion in mice. Homer 1a can attenuate cerebral ischemia-reperfusion injury.