MMP -7、MMP -9 基因多态性与结直肠癌发病的关系研究

Correlations of MMP7 and MMP9 gene polymorphisms with incidence of colorectal cancer

摘要:

目的 探讨癌胚抗原（CEA）、糖类抗原199（CA199）、基质金属蛋白酶-7（MMP -7）-181A/G、基质金属蛋白酶-9（MMP -9）P574R 基因多态性与结直肠癌发病的关系。方法 以500 例健康体检志愿者作为研究对象，按血清CEA、CA199 水平将其分为高表达组和正常组，并统计结直肠癌的发病率。采用聚合酶链反应- 限制性片段长度多态性方法分析MMP -7 基因-181A/G 位点、MMP -9 基因P574R 单核苷酸多态性分布频率，分析其与临床资料关系。结果 高表达组结直肠癌发病率高于正常组（P <0.05），高表达组MMP -7 GG+AG 基因型比例多于正常组（P <0.05），高表达组中G 等位基因频率高于正常组（P <0.05），两组MMP -9 PP、PR+RR 基因型表达比较，差异无统计学意义（P >0.05）。结直肠癌患者MMP -7 GG+AG 基因型频率高于健康人群（P <0.05），结直肠癌患者MMP -9 PR+RR、PP 基因型频率与健康人群比较，差异无统计学意义（P >0.05），MMP -7、MMP -9 基因多态性与结直肠癌TNM 分期、淋巴结转移相关（P <0.05）。结论 CEA、CA199 对结直肠癌的早期诊断有一定临床价值，MMP -7 基因-181 GG+AG、MMP-9 P574R PP基因型可能与结直肠癌的发生、发展有关。

Abstract:

Objective To investigate the associations of CEA, CA199, MMP7 gene -181A/G polymorphism, MMP9 gene P574R polymorphism with the incidence of colorectal cancer. Methods A total of 500 cases of healthy physical examination were raised and divided into high-expression group and normal group according to the serum levels of CEA and CA199, and the incidence of colorectal cancer was statistically detected. Polymerase chain reaction restriction fragment length polymorphism was used to analyze the frequency of MMP7 gene -181A/G and MMP9 gene P574R single nucleotide polymorphisms (SNPs). And the correlations of the SNPs with clinical indexes were analyzed. Results The incidence of colorectal cancer in the high-expression group was higher than that in the normal group (P < 0.05) and the patients with MMP7 GG +AG genotype were more in the high-expression group than in the normal group (P < 0.05). G allele frequency in the high-expression group was higher than that in the normal group (P < 0.05). There was no obvious difference in the MMP9 PP or PR+RR genotype expression between the two groups (P > 0.05). The frequency of MMP7 GG+AG genotype in colorectal cancer patients was higher than that in the healthy people (P < 0.05), while the frequency of MMP9 PR+RR or PP genotype was not significantly different between the patients and the healthy people (P > 0.05). MMP7 and MMP9 gene polymorphisms were associated with TNM staging and lymphatic metastasis (P < 0.05). Conclusions CA199 and CEA have some clinical value in early diagnosis of colorectal cancer. MMP7-181 GG+AG and MMP9 P574R PP genotypes may be related to the occurrence and development of colorectal cancer.