GBE 对COPD 大鼠血清和肺泡灌洗液中IL-1、IL-8 含量的影响

Effect of Ginkgo biloba extract on content of IL-1 and IL-8 in serum and bronchial alveolar lavage fluid in rat model of COPD

摘要:

目的 探讨银杏叶提取物（GBE）对慢性阻塞性肺疾病（COPD）大鼠血清和肺泡灌洗液（BALF）中白细胞介素1（IL-1）、白细胞介素8（IL-8）含量的影响。方法 将90 只清洁级Wistar 大鼠称重后，随机分为A、B、C、D、E、F 组，每组15 只。B、C、D、E、F 5 组采用烟雾吸入等复合因素复制COPD 大鼠模型；C、D 组分别在1 ～ 14 d 和29 ～ 42 d 用GBE 干预；E、F 组分别在1 ～ 14 d 和29 ～ 42 d 用红霉素干预，实验结束取各组大鼠肺动脉血和BALF，检测各组血清和BALF 中IL-1、IL-8 的含量。结果 A、C、D、E、F 组与B 组相比，血清中IL-1、IL-8 含量及BALF 中IL-1 含量低于B 组（P <0.05）。在降低血清IL-1 水平方面，早期GBE 干预优于晚期；在降低大鼠血清和BALF 中IL-1、IL-8 水平方面，GBE 与红霉素比较差异无统计学意义（P >0.05）。结论 GBE 具有抑制COPD 气道及全身炎症反应的作用。

Abstract:

Objective To study the effect of Ginkgo biloba extract (GBE) on the content of IL-1 and IL-8 in the serum and bronchial alveolar lavage fluid (BALF) in rat model of chronic obstructive pulmonary disease (COPD). Methods In this study, 90 Wistar rats were randomly divided into groups A, B, C, D, E and F with 15 rats in each group. The rat model of COPD was established by exposure to cigarette smoke and cold air stimulation composite factors in the groups B, C, D, E and F. The rats in the groups C and D were given intraperitoneal injection of GBE from day l to day l4 and day 29 to day 42 respectively. The rats in the groups E and F were given intraperitoneal injection of Erythromycin from day l to day l4 and day 29 to day 42 respectively. The serum and BALF of each group were collected after 43 days. Then the content of IL-1 and IL-8 in the serum and BALF was measured. Results Compared with the group B, the serum content of IL-1 and IL-8 and the content of IL-1 in the BALF were lower in the groups A, C, D, E and F (P < 0.05). In reducing serum IL-1 level, early GBE intervention was better than late GBE intervention.In reducing serum and BALF IL-1 and IL-8 levels, GBE had no significant difference with Erythromycin (P > 0.05). Conclusions GBE can inhibit the airway and systemic inflammation of COPD.