MMP -9 和COX -2 基因多态性与慢性阻塞性肺疾病的相关性研究

Association of MMP9 and COX2 gene polymorphisms with chronic obstructive pulmonary disease

摘要:

目的 探讨MMP- 9 和COX- 2 基因多态性与慢性阻塞性肺疾病的关系。方法 选取71 例慢性阻塞性肺疾病患者（COPD 组）和同期63 例急性呼吸道感染和健康体检者（NC 组）作为研究对象，PCRRFLP法测定MMP- 9（R279Q）、COX- 2（1195G>A）多态性，分析不同基因型与COPD 的关系。结果 NC组与COPD 组MMP- 9（R279Q）基因、COX- 2（1195G>A）的GG、AA 基因型差异有统计学意义（P =0.010和0.021），G、A 等位基因频率差异有统计学意义（P =0.000）。MMP 基因R279Q 位点的G 等位基因频率[OR=2.24（95%CI ：1.59，7.03）P =0.000]，COX- 2 基因1195G>A 位点的A 等位基因频率[OR=2.12（95%CI ：1.57，6.82）P =0.024] 为COPD 的危险因素。MMP- 9（R279Q）、COX- 2（1195G>A）基因多态性与FEV1%pred、FEV1/FVC、MMP- 9 及COX- 2 具有相关性（P =0.015、0.018、0.009、0.009，0.021、0.017、0.015 和0.000）。结论 MMP- 9 基因R279Q 位点、COX- 2 基因1195G>A 位点多态性为COPD 的危险因素，与FEV1%pred、FEV1/FVC 下降及MMP- 9、COX- 2 升高相关。

Abstract:

Objective To investigate the relationship between MMP9 and COX2 gene polymorphisms and chronic obstructive pulmonary disease. Methods A total of 71 patients with chronic obstructive pulmonary disease (COPD group) and 63 cases of acute respiratory infection and healthy persons as the objects of study. PCR-RFLP method was used to determine the polymorphisms of MMP9 (R279Q) and COX2 (1195G>A). The relationships between different genotypes and chronic obstructive pulmonary disease (COPD) were analyzed. Results Differences in MMP9 gene R279Q and GG and AA genotypes of COX2 (1195G>A) between the NC group and the COPD group had statistical significance (P = 0.010 and 0.021), the differences in the frequency of G and A alleles had statistical significance (P = 0.000). The G allele frequency at MMP9 R279Q locus (OR = 2.24, 95% CI: 1.59-7.03, P = 0.000) and the A allele frequency of COX2 gene 1195G>A locus (OR = 2.12, 95% CI: 1.57-6.82,P = 0.024) were the risk factors for COPD. MMP9 R279Q and COX2 1195G>A polymorphisms had correlations with FEV1%pred, FEV1/FVC, MMP-9 and COX-2 (P = 0.015, 0.018, 0.009, 0.009, 0.021, 0.017, 0.015 and 0.000 respectively). Conclusions MMP9 gene R279Q and COX2 gene 1195G>A polymorphisms are the independent risk factors for COPD, and correlated with decrease of FEV1%pred and FEV1/FVC and increase of MMP-9 and COX-2.