硫化氢减轻糖尿病小鼠胰岛素抵抗的作用机制研究

Hydrogen sulfide alleviates insulin resistance by reducing apoptosis of adipose cells in diabetic mice

摘要:

摘要：目的  探究硫化氢（H2S）对糖尿病小鼠胰岛素抵抗的作用和可能的分子机制。方法  C57Bl/6J小鼠随机分为正常对照组、糖尿病组、低剂量治疗组、高剂量治疗组及阳性对照组，四氧嘧啶联合高脂饮食复制糖尿病小鼠模型，检测各组小鼠脂肪组织中Caspase-3的活性、B淋巴细胞瘤-2基因（Bcl2）、Bcl-2相关X（Bax）蛋白的表达水平，以及空腹血糖、空腹血清胰岛素和血浆H2S浓度，并计算胰岛素敏感指数。结果  糖尿病小鼠Caspase-3活性和Bax表达水平下调，而Bcl2蛋白表达水平上调，治疗组各指标得到缓解。结论  H2S可以减轻胰岛素抵抗，其机制可能为减轻脂肪组织的细胞凋亡。

Abstract:

Abstract: Objective To investigate the effect of hydrogen sulfide on insulin resistance and its mechanism in mice with diabetes mellitus. Methods C57Bl6/J mice were randomly divided into normal control group, dia-betes mellitus group, low-dose therapy group, high-dose therapy group and positive control group. Diabetes mellitus models were established by high-fat diet and tetraoxypyrimidine. Caspase-3 activity, Bcl-2 and Bax expressions in adipose tissue were measured, as well as fasting plasma glucose, fasting serum insulin and hydrogen sulfide in plasma. Then, insulin sensitivity index was calculated. Results Caspase-3 activity and Bax level were down-regulated, while Bcl2 was up-regulated in mice with diabetes mellitus, and all the indexes recovered after therapy with sodium hydrosulfide. Conclusions Hydrogen sulfide could alleviate insulin resistance by reducing the apoptosis of adipose cells.