MicroRNA-101 对上皮性卵巢癌耐药细胞SKOV3/DDP 顺铂敏感性的影响

Effectofm iRNA-101 on chem osensitivity ofSKOV3/DDP cellsto Cisplatin

摘要:

探讨 MicroRNA-101（miRNA-101）对上皮性卵巢癌耐药细胞 SKOV3/DDP 顺铂敏感性的影响及相关作用机制。方法 本研究通过细胞转染技术上调 SKOV3/DDP 细胞内 miRNA-101 的表达，采用RT-PCR 检测 miRNA-101 及 BDNF mRNA 的表达情况；CCK-8 检测 SKOV3/DDP 细胞顺铂敏感性及增殖力；Annexin V-FITC/ PI 流式细胞实验检测细胞凋亡率。结果 与阴性对照组比较，miRNA-101 转染组细胞 miRNA-101 表达量及细胞凋亡率增加 [ （1.000±0.022）vs（5.380±0.246）， <0.05]，[ （11.020±0.685）%vs（26.158±1.278）%， <0.05]；BDNF mRNA 表达量降低[ （1.000±0.042）vs （0.389±0.055）， <0.05]；顺铂 IC50及细胞增殖力均下降[ （57.276±1.717）vs （33.176±2.465）， <0.05]，[ （1.776±0.030）vs （1.642±0.252）， <0.05]。结论 miRNA-101 可有效增加 SKOV3/DDP 细胞对顺铂的药物敏感性，促进细胞凋亡。

Abstract:

To explore the effectofmiRNA-101 on Cisplatin sensitivity ofchemo-resistantepithelial ovarian cancerSKOV3/DDP cellsand therelated mechanism.M ethodsIn thisstudy,theexpression ofmiRNA-101 in SKOV3/DDP cells was up-regulated by transfection.Meanwhile,RT-PCR assay was applied to detect the expressionsofmiRNA-101 mRNA and BDNF mRNA,and CCK-8 kitwasused tomeasure the Cisplatin sensitivity and proliferative ability ofSKOV3/DDP cells.In addition,the apoptosis of SKOV3/DDP cells was detected by Annexin V-FITC/PIflow cytometry.ResultsCompared with the controlgroup,the expression ofmiRNA-101 and the apoptosis rate ofmiRNA -101 in the transfected group increased significantly [(1.000 ± 0.022)vs (5.380 ±0.246), < 0.05;(11.020± 0.685)% vs(26.158 ± 1.278)% , < 0.05].The expression ofBDNF mRNA decreasedsignificantly[(1.000 ± 0.042)vs(0.389 ± 0.055), < 0.05],and the Cisplatin IC50 and the proliferative ability of SKOV3/DDP cells were obviously decreased aftertransfection [(57.276 ± 1.717)vs(33.176 ±2.465), < 0.05;(1.776 ±0.030)vs(1.642 ± 0.252), < 0.05].Conclusions miRNA-101 could effectively enhance the Cisplatin sensitivityand promotetheapoptosisofSKOV3/DDP cells.