SRPX2 在胃癌中的表达及对细胞侵袭的影响

Expression of SRPX2 in human gastric carcinoma and its role in cell invasion

摘要:

目的 探讨含sushi 重复蛋白X 连锁2（SRPX2）在人胃癌（GC）中的表达及对胃癌MGC-803细胞侵袭能力的影响。方法 收集2014 年3 月1 日-2015 年3 月1 日于该院消化内科行手术切除的45 例胃癌及对应癌旁组织。检测SRPX2 mRNA 及蛋白在胃癌及癌旁组织中的表达情况，分析胃癌组织中SRPX2 蛋白表达对肿瘤临床表型的影响。通过siRNA 下调胃癌MGC-803 细胞中SRPX2 的表达，通过Transwell 侵袭小室检测沉默SRPX2 之后MGC-803 细胞侵袭能力的变化；同时检测沉默SRPX2 后MGC-803 细胞内基质金属蛋白酶2（MMP-2）表达水平的改变。结果 SRPX2 在胃癌组织中表达升高，胃癌组织高表达SRPX2与肿瘤淋巴结转移及高TNM 分期（Ⅲ + Ⅳ期）相关（P <0.05）；沉默SRPX2 表达可抑制胃癌MGC-803细胞的侵袭能力并同时下调了MGC-803 细胞中MMP-2 的表达。结论 胃癌组织中高表达的SRPX2 与肿瘤转移表型关系密切，沉默SRPX2 可能通过下调MMP-2 的表达而抑制胃癌细胞侵袭。

Abstract:

Objective To detect the expression of Sushi Repeat Containing Protein X Linked 2 (SRPX2) in human gastric carcinoma (GC) and its role in cell invasion. Methods qRT-PCR and immunohistochemistry were applied to detect the expression of SRPX2 in GC (n = 45) and matched tumor-adjacent tissues. The correlations between SRPX2 expression and clinical features were analyzed by Pearson chi-square test. siRNA was used to silence the expression of SRPX2 in MGC-803 cells. The effect of SRPX2 on cell invasion was measured by transwell assay. MMP-2 expression was detected after silencing SRPX2 expression. Results The expression of SRPX2 was significantly up-regulated in the GC tissues, and the high expression of SRPX2 was associated with the lymphatic metastasis and advanced TNM stages (stage Ⅲ and Ⅳ ) (P < 0.05). Silencing SRPX2 could inhibit cell invasion and MMP-2 expression in the MGC-803 cells. Conclusions The expression of SRPX2 is significantly higher in GC tissues, which is correlated to tumor metastases. Silencing SRPX2 could achieve an anti-cancer role through inhibition of cell invasion.