肝龙胶囊联合水飞蓟宾胶囊在体外对大鼠肝星状细胞增殖活性的影响研究

Effect of Ganlong Capsules combined with Silibinin Capsules on in vitro proliferative activity of rat hepatic stellate cells

摘要:

研究临床常用抗肝纤维化药物水飞蓟宾胶囊单用及联合肝龙胶囊对大鼠肝星状细胞（HSC）增殖活性的影响，评价肝龙胶囊在体外对水飞蓟宾抗肝纤维化作用的影响。方法采用2 因素5 水平完全实验，单独或联合使用不同剂量水飞蓟宾胶囊和肝龙胶囊在不同时间作用于HSC，并用MTT 比色法测定HSC 增殖影响。结果水飞蓟宾胶囊与肝龙胶囊合用增强水飞蓟宾胶囊对HSC 的抑制作用，尤其是0.063、0.125 和0.250 mg/ml 浓度的水飞蓟宾胶囊与不同浓度肝龙胶囊合用后，水飞蓟宾胶囊的作用明显增加，与单用水飞蓟宾胶囊组比较差异均有统计学意义（p <0.05）。水飞蓟宾胶囊联合不同浓度肝龙胶囊的IC50 值与单用水飞蓟宾胶囊的IC50 值相比较，在24、48 和72 h时均有降低，且水飞蓟宾胶囊与不同浓度肝龙胶囊联合后，其合用指数CI<1.0，呈现出协同作用，增强了水飞蓟宾胶囊对HSC 细胞的抑制作用。结论肝龙胶囊在体外能增强水飞蓟宾胶囊的抗肝纤维化作用。

Abstract:

To investigate the effect of anti-liver fibrosis agent Silibinin Capsules alone or combined with Ganlong Capsules on proliferative activity of rat hepatic stellate cells (HSC), and to evaluate the effect of Ganlong Capsules on the in vitro anti-liver fibrosis activity of Silibinin. Methods Two-factors and five-levels full experiment was applied for single use or combined use of Silibinin Capsules with different dosages of Ganlong Capsules in HSC at different time points. Tetrazolium salt colorimetric assay (MTT) was utilized to determine the effect of Ganlong Capsules on HSC proliferation. Results Combination of Silibinin Capsules and Ganlong Capsules enhanced the suppression effect of Silibinin Capsules on HSC proliferation. The effect of Silibinin Capsules was obviously increased especially at a dosage of 0.063 mg/ml, 0.125 mg/ml or 0.250 mg/ml and in combination with Ganlong Capsules, and the differences were significant when compared with the Silibinin Capsule single drug groups (p < 0.05). Compared with the IC50 value of Silibinin Capsules alone, the IC50 values of Silibinin Capsules combined with Ganlong Capsules at different dosages decreased at 24, 48 and 72 h. Moreover, the combination indexes (CI) of the combined drug therapies were all less than 1.0, which illustrated synergistic effect and enhanced the suppression effect of Silibinin Capsules on HSC. Conclusions Ganlong Capsules can enhance the anti-liver fibrosis effect of Silibinin Capsules.