精液凝固蛋白Ⅰ活性片段的精子抑制活性分析

Analysis of sperm inhibitory activity of semenogelin I active fragment

摘要:

目的 制备重组精液凝固蛋白（Sg）及其小分子衍生肽，并分析其对精子活动的抑制作用。方法 通过分子克隆及PCR 扩增等技术得到实验用重组蛋白，从而对有正常生育能力的男性精子活动进行抑制实验分析。结果 在生理条件下，精液Sg 的N 和C 端片段均对精子运动存在抑制作用，其中以N 端片段更为显著。结论 精液Sg 中存在能够抑制精子运动的氨基酸片段，而Sg24 ～ 163 的作用最为显著。

Abstract:

Objectlve To prepare the recombinant semenogelin (Sg) and its different small molecule derived peptides and study the effect of Sg on sperm motility. Methods Molecular cloning and PCR amplification were used to prepare the recombinant Sg, then the recombinant protein was used to test the activity of male sperm with normal reproductive function. Results At the concentration of 200 pg/sperm, the N-terminal fragment of the recombinant Sg had the effect of inhibiting sperm motility (P < 0.05), and the C-terminal fragment also inhibited sperm motility (P < 0.05). Conclusions There are amino acid fragments in the Sg molecule that can inhibit sperm motility, Sg24-163 has the highest sperm inhibitory activity.