结肠癌血清microRNA 表达谱的检测及临床应用价值

Detection of serum microRNA profile in colon cancer and its clinical application value

摘要:

目的 找寻潜在的新型的结肠癌血清microRNA（miRNA）表达谱，探讨其临床应用价值。方法miRNA 微阵列芯片技术检测结肠癌患者和正常人血清中miRNA 表达的差异，并对有差异表达的miRNA 在60 例结肠癌患者血清和60 例正常对照组血清中的表达进行实时荧光定量聚合酶链反应（qRT-PCR）验证。结果①芯片杂交结果中共有87 种miRNAs 在结肠癌患者血清和正常人血清标本中有差异表达，其中上调表达有39 个，下调表达有48 个。② qRT-PCR 对miRNAs 进行验证，其中miR-31、miR-141、miR-224-3p、miR-576-5p 和miR-4669 这5 个miR 分子在结肠癌症患者中的表达相对于正常对照组差异具有统计学意义（P <0.05）。③ miR-31、miR-141、miR-224-3p、miR-576-5p 和miR-4669 这5 个miR 分子组成的miR- 表达谱诊断结肠癌的效率较高（ROC 曲线下面积为0.992）。结论 临床检测结肠癌患者miR-31、miR-141、miR-224-3p、miR-576-5p 和miR-4669 循环分子标志物表达谱有助于诊断结肠癌，有望成为结肠癌患者临床诊疗评估的重要指标。

Abstract:

Objective To explore the potential novel serum microRNA (miRNA) profile in colon cancer and discuss its clinical application value. Methods The difference of serum miRNA expression between colon cancer patients and healthy people was detected by miRNA microarray chip technology. The differential expression of miRNAs in serum of 60 cases of colon cancer and 60 normal people were validated by qRT-PCR. Results A total of 87 miRNAs were differentially expressed in the serum of the colon cancer patients compared to the normal people, among which 39 miRNAs were upregulated and 48 miRNAs were down-regulated. miR-31, miR-141, miR-224-3p, miR-576-5p and miR-4669 expressions in the colon cancer patients were significantly different from those in the normal control group (P < 0.05). MicroRNA profile (including miR-31, miR-141, miR-224-3p,miR-576-5p and miR-4669) has a higher efficiency in diagnosis of colon cancer (area under the ROC curve was 0.992). Conclusions Clinical detection of expression profile of circulating molecular markers miR-31, miR-141,miR-224-3p, miR-576-5p and miR-4669 in colon cancer patients is useful for the diagnosis of colon cancer. It is expected to be an important index for evaluation of clinical diagnosis and treatment in patients with colon cancer.