FGF-1 对TGF-β1 诱导的H9c2 心肌细胞凋亡与Caspase-3，Caspase-8 和Caspase-9 表达的关系研究

Effect of FGF-1 on caspase-3, caspase-8 and caspase-9 expressions and apoptosis of myocardial H9c2 cells induced by TGF-β1

摘要:

目的 观察酸性成纤维细胞生长因子（FGF-1）对转化生长因子-β1（TGF-β1）诱导的H9c2心肌细胞凋亡作用及对半胱氨酸蛋白酶-3（Caspase-3），半胱氨酸蛋白酶-8（Caspase-8）和半胱氨酸蛋白酶-9（Caspase-9）表达的影响，并探讨可能机制。方法 体外培养H9c2 细胞，随机分为5 组：模型组，肝素（Heparin）组、FGF-1 组、FGF-1/Heparin 组和正常对照组。采用3-（4，5- 二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）比色分析检测H9c2 细胞的存活率，TUNEL 染色检测H9c2 细胞凋亡指数和Western blot 分析检测H9c2 细胞Caspase-3，Caspase-8 和Caspase-9 的表达。结果 模型组H9c2 细胞存活率与正常对照组比较，差异有统计学意义（P <0.05），模型组降低；FGF-1 组和FGF-1/Heparin 组H9c2 细胞存活率与模型组比较，差异有统计学意义（P <0.05），FGF-1 组和FGF-1/Heparin 组均升高。模型组H9c2 细胞凋亡指数，Caspase-3、Caspase-8 和Caspase-9 的表达与正常对照组比较，差异有统计学意义（P <0.05），模型组升高；FGF-1 组和FGF-1/Heparin 组H9c2 细胞凋亡指数，Caspase-3、Caspase-8 和Caspase-9 的表达与模型组比较，差异有统计学意义（P <0.05），FGF-1 组和FGF-1/Heparin 组降低。结论 FGF-1 对TGF-β1诱导的H9c2 心肌细胞凋亡具有抑制作用，其机制可能与抑制Caspase-3、Caspase-8 和Caspase-9 的表达有关。

Abstract:

Objective To observe the effect of acidic fibroblast growth factor (FGF-1) on the apoptosis of myocardial H9c2 cells induced by transforming growth factor-β1 (TGF-β1) and the expressions of cysteinyl aspartate specific proteinase-3 (caspase-3), caspase-8 and caspase-9 and to discuss its possible mechanisms. Methods H9c2 cells were cultured and randomly divided into 5 groups, i.e. model group, Heparin group, FGF-1 group, FGF-1/Heparin group and normal control group. The survival rate of H9c2 cells was measured by 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The apoptotic index of H9c2 cells was observed by TUNEL staining. The expression levels of caspase-3, caspase-8 and caspase-9 were detected by Western blot. Results Compared with the normal control group, the survival rate of H9c2 cells was significantly decreased in the model group (P < 0.05). Compared with the model group, the survival rate of H9c2 cells was significantly increased in the FGF-1 group and the FGF-1/Heparin group (P < 0.05). Compared with the normal control group, the apoptosis index and the expressions of caspase-3, caspase-8 and caspase-9 of H9c2 cells in the model group were significantly increased (P < 0.05). Compared with the model group, the apoptosis index and the expressions of caspase-3, caspase-8 and caspase-9 of H9c2 cells were significantly decreased in theFGF-1 group and the FGF-1/Heparin group (P < 0.05). Conclusions FGF-1 could obviously inhibit the apoptosis of H9c2 cells induced by TGF-β1 and its possible mechanism may be related to decrease of the expression level of caspase-3, caspase-8 and caspase-9.