白藜芦醇对单个核细胞氧化应激的作用和影响

Effect of resveratrol on oxidative stress in peripheral blood mononuclear cells of hyperoxia-exposed preterm infants

摘要:

摘要：目的  探讨高氧暴露早产儿外周血单个核细胞应用白藜芦醇对氧化应激的影响。方法  52例早产儿随机分为白藜芦醇+高氧组和高氧组，每组26例，选取同期吸入常压空气早产儿20例作为对照组。抽取患儿桡动脉血2 ml，采用Ficoll密度梯度离心法分离单个核细胞。观察3组单个核细胞活性氧水平、培养液丙二醛（MDA）水平、细胞自噬体、血红细胞加氧酶（HO-1）蛋白水平及活性。结果  3组活性氧、MDA、细胞自噬体、HO-1蛋白水平、HO-1活性比较，差异有统计学意义（P <0.05），高氧组活性氧、MDA、细胞自噬体、HO-1蛋白水平、HO-1活性高于对照组和白藜芦醇+高氧组；白藜芦醇+高氧组活性氧、MDA、细胞自噬体、HO-1蛋白水平、HO-1活性高于对照组。结论  白藜芦醇能够有效对抗高氧暴露产生的氧化应激，减少氧化应激损伤。

Abstract:

Abstract: Objective To investigate the effect of resveratrol on oxidative stress in peripheral blood mononuclear cells of hyperoxia-exposed premature infants. Methods Fifty-two cases of premature infants were randomly divided into resveratrol plus high oxygen group and high oxygen group with 26 cases in each group, and 20 preterm infants with normal atmospheric air inhalation during the same period were selected as control group. Each infant was drawn 2 ml of blood from the radial artery, and then mononuclear cells were isolated by Ficoll density gradient centrifugation. The levels of reactive oxygen species and malondialdehyde (MDA) in the culture medium, autophagosomes and the level and activity of erythrocyte oxygenase (HO-1) protein were observed. Results The active oxygen, MDA, autophagosomes, HO-1 protein level and HO-1 activity were signi-ficantly different among the 3 groups (P < 0.05), which were the highest in the high oxygen group and lowest in the control group. Conclusions Resveratrol can effectively protect premature infants from oxidative stress injury induced by hyperoxia exposure.