呼出气冷凝液中LTB4和CCL11水平与儿童哮喘分期的研究

Role of LTB4 and CCL11 levels of exhaled breath condensate in assessment of asthma in children

摘要:

摘要：目的  探索呼出气冷凝液（EBC）中白三烯B4（LTB4）和嗜酸性粒细胞趋化因子（CCL11）2种标记物对儿童哮喘病情的评估作用。方法  收集64例哮喘患儿。其中，急性发作期33例，慢性持续期18例，缓解期13例，选同时期8例健康儿童作对照组。采用R-Tube收集每个实验对象的EBC，酶联免疫吸附法检测每个标本中LTB4和CCL11。结果  ①EBC中LTB4水平从高到低依次为发作组（70.89±8.31）pg/ml、持续组（35.27±6.61）pg/ml、缓解组（24.58±4.90）pg/ml、对照组（15.53±3.24）pg/ml，各组间比较，差异有统计学意义（P <0.05）；②EBC中CCL11水平最高为哮喘发作组（3.38±0.65）pg/ml，其次为持续组（2.46±0.52）pg/ml、缓解组（0.90±0.36）pg/ml和对照组（0.71±0.26）pg/ml，缓解组与对照组比较，差异无统计学意义。结论  EBC中LTB4、CCL11水平能反映哮喘气道炎症及其控制情况，对哮喘患儿病情的评估有一定价值。

Abstract:

Abstract: Objective To investigate effect of leukotriene B4 (LTB4) and eotaxin (CCL11) in exhaled breath condensate (EBC) on the evaluation of asthma in children. Methods Sixty-four children with asthma were enrolled, including 33 with acute asthma exacerbation, 18 with chronic asthma and 13 with asthma remission; and 8 healthy children were enrolled as controls. The EBC of each subject was collected using R-Tube. LTB4 and CCL11 were detected by ELISA in each sample. Results The LTB4 levels of EBC in the order from high to low were the acute asthma exacerbation group [(70.89 ± 8.31) pg/ml], the chronic asthma group [(35.27 ± 6.61) pg/ml], the asthma remission group [(24.58 ± 4.90 pg/ml)], and the control group [(15.53 ± 3.24 pg/ml)]; there were significant differences among the groups (P < 0.05). The CCL11 level of EBC was the highest in the acute asthma exacerbation group [(3.38 ± 0.65) pg/ml], followed by the chronic asthma group [(2.46 ± 0.52) pg/ml], the asthma remission group [(0.90 ± 0.36) pg/ml], and the control group [(0.71 ± 0.26) pg/ml]; there was no significant difference between the asthma remission group and the control group (P > 0.05). Conclusions The levels of LTB4 and CCL11 in EBC can reflect the status of airway inflammation in asthma and its control. They are valuable in assessment of the condition of children with asthma.