芪参益气滴丸对大鼠心肌缺血再灌注损伤的作用机制研究

Protective effect of Qishenyiqi Dripping Pills on rats with myocardial ischemia-reperfusion injury

摘要:

探讨芪参益气滴丸（QS）对大鼠心肌缺血再灌注时，心肌细胞尿路上皮癌胚抗原1（UCA1）长链非编码RNA、凋亡及凋亡相关基因表达的影响。方法结扎SD大鼠冠状动脉左前降支，造成心肌缺血40 min后剪断结扎线，再灌注120 min，复制实验性心肌缺血再灌注模型，将36 只大鼠随机分为假手术组（只穿刺不结扎，Control 组）、缺血再灌注组（I/R 组）、QS 干预组（QS+I/R 组），术后采用原位末端标记法检测心肌细胞凋亡指数，采用荧光定量聚合酶链反应分析并检测3 组大鼠心脏组织中UCA1 的表达，Western blot 检测p27 蛋白表达水平。结果QS+I/R组的细胞凋亡数与I/R 组比较，差异有统计学意义（p <0.05）；与I/R 组比较，QS+I/R 组UCA1 整体表达水平上升，而p27 蛋白减少（ p<0.05）；并且UCA1 与p27 蛋白的表达呈负相关。结论QS 能降低大鼠缺血再灌注心肌细胞凋亡率，其可能通过上调UCA1 水平，减少p27 蛋白表达，保护损伤的心肌细胞。

Abstract:

To investigate the effect of Qishenyiqi Dripping Pills on urothelial carcinoma antigen 1 (UCA1) LncRNA, apoptosis and apoptosis related gene expression of myocardial cells in rats with myocardial ischemia reperfusion. Methods Ischemia reperfusion injury model of SD rats was established by ligating left anterior descending branch of coronary artery for 40 min followed by reperfusion for 120 min. Thirty-six rats were randomly divided into sham operation group (only puncture wihout ligation), ischemia-reperfusion group (IR group) and Qishenyiqi intervention group (QS+IR group). After operation, the apoptosis index was detected by TUNEL method; fluorescence quantitative PCR technique and Western blot were used to detect the expression levels of UCA1 and p27 protein respectively. Results The number of apoptosis in the QS+IR group was significantly smaller than that in the IR group ( p< 0.05). The overall level of UCA1 expression significantly increased and p27 protein markedly decreased in the QS+IR group compared with the IR group (p < 0.05); and the expression of UCA1 was negatively correlated with the expression of p27 protein. Conclusions Qishenyiqi Dripping Pills can reduce myocardial apoptosis rate in rats with myocardial ischemia and reperfusion, which may protect the injured myocardial cells by increasing the level of UCA1 and reducing the expression of p27protein.