胃癌中高表达的CBX7对细胞迁移、侵袭的影响

CBX7 expression in human gastric cancer and its role in cell migration and invasion

摘要:

探究人胃癌组织中染色盒同源物7（CBX7）的表达及对胃癌细胞侵袭能力的影响。方法收集2013 年1 月1 日-2015 年1 月1 日间于该院普通外科行手术切除的45 例胃癌及对应癌旁组织，免疫组织化学染色观察CBX7蛋白表达，分析胃癌组织中CBX7 蛋白表达高低与肿瘤临床特征的关系。通过重组质粒在胃癌SGC-7901 细胞中过表达CBX7，经Transwell 小室实验确定CBX7 过表达对胃癌细胞迁移侵袭的作用。结果CBX7 在胃癌组织中表达较癌旁组织下降（ t=3.517， p=0.000），胃癌组织低表达CBX7 与肿瘤淋巴结转移（χ2=5.829，p =0.022）及高TNM 分期（Ⅲ+Ⅳ期，χ2=4.465， p=0.047）相关。过表达CBX7 对SGC-7901 的迁移（t =42.040，p =0.000）及侵袭（ t=40.119，p =0.000）具有显著的削弱作用。结论CBX7 在胃癌组织中表达降低，过表达CBX7 能够通过抑制胃癌细胞迁移及侵袭发挥抗肿瘤作用。

Abstract:

To study the expression of chromobox homolog 7 (CBX7) in human gastric carcinoma and its role in cell migration and invasion. Methods Immunohistochemical method was applied to detect the expression of CBX7 in gastric cancer and matched tumor-adjacent tissues of 45 patients. The correlations between CBX7 expression and clinical features were analyzed by Pearson chi-square test. Recombinant CBX7 plasmid was used to over-express CBX7 in SGC-7901 cells. The effect of CBX7 on cell migration and invasion was measured by Transwell assay. Results The expression of CBX7 was significantly down-regulated in the gastric cancer tissues, and the low expression of CBX7 was associated with lymphatic metastasis and advanced TNM stage (stageⅢand Ⅳ)(p < 0.05). Over -expression of CBX7 inhibited the migration and invasion of SGC -7901 cells (p < 0.05). Conclusions CBX7 expression is decreased in gastric cancer tissues. Up-regulation of CBX7 expression could play an anti-cancer role through inhibition of cell migration and invasion.