奈帕芬胺对角膜组织中前列腺素E2的作用研究

Effect of Nepafenac on prostaglandin E2 in corneal tissue

摘要:

目的通过复制兔眼前节缺血（ASI）模型，检测模型角膜中前列腺素E2（PGE2）的表达，探讨奈帕芬胺在角膜组织中对PGE2 的抑制作用。方法将54 只已排除眼疾的健康雄性纯种大耳白兔随机分成对照组（18 只兔，36 只眼）和手术组（36 只兔，72 只眼）。手术组36 只兔的左眼（36 只眼）为缺血组，右眼（36 只眼）为治疗组。不同时间在裂隙灯显微镜下观察各组角膜的变化，然后取角膜做病理学检查和免疫组织化学检查，观察各时间PGE2 在角膜中的表达。结果缺血组各时间点PGE2 表达增高（p <0.05），治疗组PGE2 表达较缺血组下降（p <0.05）。结论在ASI 的发病过程中，PGE2 发挥重要作用，奈帕芬胺在角膜组织中可有效抑制PGE2。

Abstract:

Objective To set up a rabbit model of anterior segment ischemia (ASI) with the method of multi-rectus tenotomy, and to investigate the expression of PGE2 in cornea of the model and the effect of Nepafenac on corneal PGE2. Methods Fifty-four purebred health male Japanese White Rabbits without eye diseases were randomly divided into two groups. There were 18 rabbits (36 eyes) in the control group and 36 rabbits (72 eyes) in the operation group which were futher randomly divided into ischemic group (36 left eyes of the 36 rabbits) and treatment group (36 right eyes of the 36 rabbits). The changes of cornea were observed under slit-lamp in different periods in each group. Then the cornea tissue was taken for pathological and immunohistochemical examinations, the expression of PGE2 in cornea tissue was observed at different time. Results At each time point, the expression of PGE2 in the ischemic group increased (p < 0.05), the expression of PGE2 in the treatment group reduced compared to the ischemia group with statistical significance (p < 0.05). Conclusions PGE2 may play an important role during the onset of ASI. Nepafenac can effectively restrain PGE2 in the corneal tissue.