苦碟子注射液对大鼠肾脏缺血再灌注损伤的影响

Effect of Kudiezi injection on renal ischemia-reperfusion injury in rats

摘要:

目的 观察苦碟子注射液对大鼠肾脏缺血再灌注损伤的影响并探讨其可能机制。方法 将18 只健康雄性SD 大鼠随机分为假手术组（Sham 组）、模型组（IRI 组）、苦碟子注射液组（KDZ 组）。采用全自动生化分析仪检测血清肌酐（Scr）、尿素氮（BUN），采用检测试剂盒分别检测肾组织中超氧化物歧化酶（SOD）、丙二醛（MDA）活力，HE 染色观察病理组织学变化，免疫组织化学法观察肾组织中白细胞介素6（IL-6）表达情况，ELISA 检测肾组织细胞间黏附分子-1（ICAM-1）的含量。结果 与Sham 组相比，IRI 组Scr 及 BUN 含量和肾组织MDA 含量均升高，KDZ 组Scr 及BUN 含量和肾组织MDA 含量均介于Sham 组与IRI组之间；IRI 组肾组织SOD 活性较Sham 组降低，KDZ 组SOD 活性介于Sham 组与IRI 组之间。HE 染色发现Sham 组肾小管形态结构基本正常，管腔内仅有极少量上皮细胞脱落，间质内可见少量炎症细胞浸润；IRI组肾小管上皮细胞出现空泡变性，管腔缩小甚至堵塞，其内可见脱落细胞和管型，间质水肿明显，其内可见大量炎症细胞浸润；KDZ 组肾小管上皮细胞变性不明显，管腔内可见极少量上皮细胞和管型，间质轻度水肿，可见少量炎症细胞浸润，KDZ 组病理损伤程度轻于IRI 组。免疫组织化学发现Sham 组有少量IL-6 表达，IRI 组IL-6 表达量较Sham 组增多，KDZ 组表达量较IRI 组减少。ELISA 检测发现IRI 组、KDZ 组ICAM-1含量均较Sham 组增高，但KDZ 组低于IRI 组。结论 苦碟子注射液能够减轻大鼠缺血再灌注损伤，其机制与其抗氧化应激、减轻炎症损伤有关。

Abstract:

Objective To observe the effect of Kudiezi injection on renal ischemia-reperfusion injury in rats and to investigate its possible mechanisms. Methods A total of 18 male Sprasue-Dawley (SD) rats were randomly divided into three groups: sham group, model group (IRI group), and Kudiezi injection group (KDZ group). Serum creatinine (Scr) and blood urea nitrogen (BUN) were detected by automatic biochemistry analyzer. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in the renal tissue was respectively assessed by relevant detection kits. Histopathological changes were observed after HE staining. The expression of interleukin 6 (IL-6) in the renal tissue was checked by immunohistochemistry. The content of intercellular adhesion molecule-1 (ICAM-1) was tested by ELISA. Results The levels of Scr, BUN and MDA in the renal tissue of the IRI group were significantly increased compared with the sham group, and the levels of Scr and BUN and MDA of the renal tssue in the KDZ group were between those in the sham group and the IRI group. The activity of SOD in the renal tissue of the IRI group was significantly lower than that of the sham group, while the activity of SOD in the renal tissue of the KDZ group was between that of the sham group and the IRI group. HE staining showed that the morphology and structure of renal tubules in the sham group were basically normal, only a very small number of shedding epithelial cells were observed in the lumen and a small amount of inflammatory cell infiltration was noted in the stroma. In the IRI group, vacuolar degeneration of renal tubular epithelial cells was seen, the tubular lumen became narrow even blocked, there were shedding cells and tubular casts in the lumen and evident stromal edema as well as obvious inflammatory cell infiltration in the stroma. In the KDZ group, degeneration of renal tubular epithelial cells was not apperent, a very small quantity of epithelial cells and tubular casts were noted in the lumen, slight stromal edema and slight inflammatory cell infiltration in the stroma were observed. The degree of pathological injury in the KDZ group was significantly lower than that in the IRI group. Immunohistochemistry showed that the expression of IL-6 in the sham group was very low, the expression of IL-6 in the IRI group was evidently higher than that in the sham group, and the expression of IL-6 in the KDZ group was evidently lower than that in the IRI group. ELISA showed that the content of ICAM-1 in both IRI and KDZ groups was higher than that in the sham group, but the content of ICAM-1 in the KDZ group was lower than that in the IRI group. Conclusions Kudiezi injection can reduce renal ischemia-reperfusion injury in rats, the mechanism is related to the inhibition of oxidative stress and reduction of inflammatory injury.