Bcl -2 基因沉默对胃腺癌SGC-7901 细胞5-Fu 敏感性的影响

Impact of BCL2 gene silencing on 5-Fu sensitivity in gastric adenocarcinoma SGC-7901 cells

摘要:

目的 探讨B 淋巴细胞瘤-2（Bcl -2）基因沉默增强胃腺癌SGC-7901 细胞对5- 氟尿嘧啶（5-FU）敏感性的价值。方法 设立人胃腺癌SGC-7901 细胞研究组和对照组，采用逆转录聚合酶链反应（RTPCR）检测两组人胃腺癌SGC-7901 细胞中Bcl-2 mRNA 的表达。研究组采用脂质体法转染化学合成的Bcl-2 siRNA 序列至人胃腺癌SGC-7901 细胞，对照组不进行干预。比较转染前后两组Bcl-2 mRNA 的表达水平。两组均添加不同浓度5-FU，采用Annexin V 标记和流式细胞仪检测48 h 后两组不同浓度5-FU 细胞株的增殖抑制率和凋亡率。结果 两组转染前Bcl-2 mRNA 相对表达量比较，差异无统计学意义（P >0.05）。与转染前比较，研究组转染后的Bcl-2 mRNA 相对表达量降低（P <0.05）；与对照组比较，研究组转染后的Bcl-2mRNA 相对表达量降低（P <0.05）。与对照组比较，研究组培养48 h 的细胞增殖抑制率和凋亡率较高（P <0.05）。结论 Bcl -2 基因沉默可增强胃腺癌SGC-7901 细胞对5-FU 的敏感性，抑制癌细胞的增殖，并促进癌细胞的凋亡。Bcl -2 基因沉默可能是改善胃腺癌5-FU 疗效的有效方法。

关键词:B 淋巴细胞瘤-2 ;基因沉默;增强;胃腺癌;SGC-7901 细胞;5- 氟尿嘧啶;敏感性

Abstract:

Objective To explore the value of B cell lymphoma 2 (BCL2 ) gene silencing in enhancing 5-fluorouracil (5-FU) sensitivity in gastric adenocarcinoma SGC-7901 cells. Methods Human gastric adenocarcinoma SGC-7901 cells were set up as research group and control group. In the research group, chemicallysynthesized BCL2 siRNA sequence was transfected into gastric adenocarcinoma SGC-7901 cells by liposome method;while the control group had no relative intervention. RT-PCR was applied in detection of BCL2 mRNA expression in gastric adenocarcinoma SGC-7901 cells of the two groups before and after transfection. Both groups were added with different concentrations of 5-FU and tagged with Annexin V; 48 h after adding 5-FU flow cytometry was applied to detect cell proliferation inhibition rate and apoptosis rate. Results Before transfection the relative expression of BCL2 mRNA had no statistical difference between the two groups (P > 0.05). The relative expression of BCL2 mRNA in the research group decreased after transfection compared to that before transfection and that in the control group (P < 0.05). After cell culture for 48 h, the proliferation inhibition and apoptosis rates of the research group were higher than those of the control group (P < 0.05). Conclusions BCL2 gene silencing can enhance 5-FU sensitivity in gastric adenocarcinoma SGC-7901 cells, inhibit cancer cell proliferation and promote cancer cell apoptosis; therefore, BCL2 gene silencing may be an effective way to enhance the effect of 5-FU therapy for gastric adenocarcinoma.

Key words:B cell lymphoma 2; gene silencing; enhance; gastric adenocarcinoma; SGC-7901 cell;5-fluorouracil; sensitivity