DAT和D2R在垂体腺瘤组织中的表达及其意义

Expressions and significance of DAT and D2R in pituitary adenoma tissues

摘要:

目的探讨垂体腺瘤组织中多巴胺转运体（DAT）和多巴胺D2受体（D2R）的表达变化及其意义。方法选取179 例手术治疗的垂体腺瘤患者，根据术前内分泌激素检查和术后病理学检查结果，将患者分为泌乳腺瘤（PRL）45 例、生长激素腺瘤（GH）43 例、促肾上腺皮质激素腺瘤（ACTH）32 例及无功能垂体腺瘤（NFPA）59例，免疫组织化学法检测垂体腺瘤组织和瘤旁正常组织中DAT、D2R的表达，分析D2R、DAT 在垂体腺瘤组织中表达的相关性。结果PRL、GH及ACTH组织中D2R、DAT 表达高于NFPA 和瘤旁正常组织，PRL 和GH 组织中D2R、DAT 表达高于ACTH 组织（p <0.05）；D2R、DAT 在垂体腺瘤组织中的表达与性别、年龄、肿瘤直径、肿瘤质地、KNOSP 分级、是否侵袭及是否复发无关（p >0.05）；Spearman 相关分析显示，D2R 在垂体腺瘤组织中的表达与DAT 呈正相关（r =0.571，p =0.000）。结论D2R、DAT 在PRL 和GH腺瘤组织中呈高表达，而在ACTH 和NFPA 腺瘤中组织呈低表达，提示多巴胺代谢紊乱可能参与不同病理学类型垂体腺瘤的发生。

Abstract:

Objective To investigate the changes of expressions of dopamine transporter (DAT) and dopamine D2 receptor (D2R) in pituitary adenomas and their significance. Methods In this study, 179 cases of patients with pituitary adenomas undergoing surgical treatment were selected. According to the results of preoperative hormone examination and postoperative pathologic test, the patients were divided into prolactinoma(PRL,n = 45), growth hormone adenoma (GH, n= 43), adrenocorticotropic adenoma (ACTH, n= 32) and nonfunctioning pituitary adenoma (NFPA,n = 59) groups. The expressions of DAT and D2R in the pituitary adenomas and the adjacent normal tissues were detected using immunohistochemistry. The correlations between the expressions of D2R and DAT in the pituitary adenoma tissues were analyzed by Spearman correlation analysis. Results Qualitative and semi-quantitative analysis results showed that, the expressions of D2R and DAT in the PRL, GH and ACTH tissues were higher than those in the NFPA and adjacent normal tissues,and the expressions of D2R and DAT in the PRL and GH tissues were higher than those in the ACTH tissues, the differences were statistically significant (p < 0.05). The expressions of D2R and DAT in the pituitary adenoma tissues were not related with gender, age, tumor diameter, tumor texture, KNOSP classification, tumor invasion or recurrence (p > 0.05). Spearman correlation analysis showed that the expression of D2R in the pituitary adenoma tissues was positively correlated with the expression of DAT (r = 0.571,p =0.000). Conclusions D2R and DAT are highly expressed in PRL and GH adenoma tissues, but lowly expressed in ACTH and NFPA tissues. It suggested that dopamine metabolic disorders may be involved in the pathogenesis of different types of pituitary adenomas.