鞣质联合环丙沙星对鲍曼不动杆菌生物膜形成的影响

Tannin combination with Ciprofloxacin could effect on biofilm of Aacinetobacter baumanni

摘要:

目的研究石榴皮提取物鞣质联合环丙沙星（CIP）对鲍曼不动杆菌（Ab）生物膜形成的影响。方法采用2 倍稀释法测得石榴皮提取物鞣质和CIP 对Ab ATCC19606 的最低抑菌浓度（MIC）；将1/2MIC、MIC、2MIC浓度的鞣质和CIP单独及联合用药作用于Ab生物膜4、8、24 和48 h，采用结晶紫法和荧光显微镜法观察生物膜的形成。结果鞣质和CIP 对Ab 的MIC 值为1.95 和0.50 mg/ml；联合用药较单独用药对Ab 生物膜的形成影响大，且2MIC鞣质+2MIC CIP联合作用24 h，对生物膜形成影响最大。结论石榴皮提取物鞣质和 CIP联合用药对生物膜形成的影响比单独用药明显，且2MIC鞣质+2CIP联合用药对Ab生物膜作用24 h影响效果最佳。

Abstract:

Objective To explore the effect of tannin extracted from Pomegranate rind combined with Ciprofloxacin (CIP) on the biofilm of Aacinetobacter baumanni(Ab). Methods The minimal inhibitory concentrations (MIC) of tannin and CIP to Ab ATCC19606 were determined by broth dilution method. The 1/2MIC, MIC and 2MIC of tannin, CIP and their combination acted on Ab for 4, 8, 24 and 48 h,respectively; then the biofilm formation of Ab was observed by crystal violet staining assay and fluorescence microscopy. Results The MIC of tannin and CIP to Ab ATCC19606 were 1.95 mg/ml and 0.50 mg/ml respectively. The effect of tannin or CIP separately on the biofilm of Ab was weaker than that of their combination. And their combination could have some effect on the biofilms, of which the combined use of 2MIC tannin and 2MIC CIP for 24 h had the greatest effect on biofilm formation of Ab. Conclusions Combined use of tannin extracted from Pomegranate rind and CIP has more obvious effect on the biofilm formation of Ab, moreover, the combination of 2MIC tannin and 2MIC CIP for 24 h has the best effect.