结肠癌患者ΔNp63和NF-资B的表达及意义

Expression of △Np63 and NF-kB in colon cancer patients and significance

摘要:

目的探讨结肠癌患者N末端截短型63 基因（ΔNp63）和核转录因子资B（NF-资B）的表达及意义。方法采用免疫组织化学法检测结肠癌患者ΔNp63 和NF-资B 的表达；采用实时荧光定量聚合酶链反应（qRT-PCR）和Western blot 检测ΔNp63 和NF-资B 信使核糖核酸（mRNA）和蛋白的表达；采用半胱氨酸的天冬氨酸蛋白水解酶3（Caspase-3）活性检测试剂盒检测凋亡蛋白Caspase-3 表达。结果与对照组比较，结肠癌患者ΔNp63 蛋白表达升高35.57%，NF-资B 蛋白表达升高58.93%，差异有统计学意义（p <0.05）；结肠癌患者ΔNp63 和NF-资B mRNA 和蛋白表达均升高，差异有统计学意义（p <0.05）；结肠癌患者Caspase-3 活性升高，差异有统计学意义（p <0.05）。结论结肠癌患者ΔNp63 和NF-资B 呈过度表达状态；ΔNp63 和NF-资B的过表达在结肠癌发生、发展机制中可能起到重要作用。

Abstract:

Objective To investigate the expression and effect of △Np63 and NF-kB in patients with colon cancer. Methods The expressions of △Np63 and NF-kB in patients with colon cancer were detected by immunohistochemical method. The mRNA and protein expressed by △Np63 and NF-kB were detected by real-time polymerase chain reaction (RT-PCR) and Western blot. The expression of Caspase-3 in apoptotic cells was detected by Caspase-3 activity assay kit. Results Compared with the control group, the level of protein expressed by △Np63 rose by 35.57% and the level of protein expressed by NF-kB rose by 58.93%,the difference was statistically significant (p < 0.05). The mRNA and protein expressed by △Np63 and NF-kB were obviously up-regulated, the differences were statistically significant (p < 0.05). The activity of Caspase-3 in the colon cancer group was obviously increased, the difference was statistically significant (p < 0.05). Conclusions The expressions of △Np63 and NF-kB in colon cancer patients are over-expressed, and the over-expressed △Np63 and NF-kB may play important roles in the development and progression of colon cancer.