二甲双胍对糖尿病大鼠肾脏的保护作用及其机制研究

Protective effect of Metformin on Streptozotocin-induced nephropathy in diabetic rats

摘要:

目的观察二甲双胍对链脲佐菌素（STZ）诱导糖尿病肾病大鼠肾脏的影响，分析二甲双胍的防治功效及其机制。方法选取健康雄性14 周龄Wistar 大鼠36 只，随机分为3 组：正常对照组、糖尿病模型组和二甲双胍干预组。利用STZ 诱导大鼠糖尿病，正常对照组给予生理盐水，第8 周时大鼠糖尿病肾病模型复制成功。采用Western blot检测各组大鼠肾脏中炎症细胞因子[肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）、白介素6（IL-6）]和抗炎症细胞因子[白介素10（IL-10）]的表达。结果糖尿病模型组TNF-α、IL-1β及IL-6的表达水平高于正常对照组（p <0.05），IL-10 的表达水平低于正常对照组（p <0.05）；给予二甲双胍干预后，上述结果逆（p <0.05）。结论二甲双胍可通过抑制肾脏炎症细胞因子的表达，保护STZ诱导的糖尿病大鼠的肾脏。

Abstract:

Objective To observe the effect of Metformin on diabetic nephropathy induced by Streptozotocin in rats, and to explore its preventive and therapeutic effect on diabetic nephropathy. Methods Thirty-six healthy male 14-week-old Wistar rats were randomly divided into three groups, i.e. normal control group, diabetic group and Metformin intervention group. Streptozotocin was used to induce diabetic rat model.TNF-α, IL-1β, IL-6 and anti-inflammatory cytokine IL-10 in the renal tissues were detected by Western blot. Results The levels of TNF-α, IL-6 and IL-1β in the renal tissues of the diabetic rats were significantly higher than those in the normal controls, while the IL-10 level was significantly lower than that in the control group (p < 0.05). After treatment with Metformin, the levels of TNF-α, IL-6 and IL-1β in the renal tissues decreased while the IL-10 level increased in the diabetic rats (p < 0.05). Conclusions Metformin can ameliorate diabetic nephropathy induced by Streptozotocin in rats by inhibiting the expression of inflammatory cytokines.