银杏叶提取物对COPD大鼠肺组织超微结构的影响

Effect of Ginkgo biloba extract on pulmonary ultrastructures of rats with chronic obstructive pulmonary disease

摘要:

目的研究银杏叶提取物（GBE）对慢性阻塞性肺疾病（COPD）大鼠肺组织超微结构的影响。方法将清洁级7 周龄雄性Wistar大鼠90 只称重，随机分组为正常对照组（A 组）、COPD 对照组（B 组）、银杏叶提取物早期干预组（C 组）、银杏叶提取物后期干预组（D 组）、红霉素早期干预组（E 组）、红霉素后期干预组（F），并复制模型。每组随机选取6 只，利用透射电镜，观察各组大鼠肺组织超微结构改变，计算线粒体病变率。结果B、C、D、E、F 组有COPD特征性改变，但程度不同。电镜下观察各组较B组结构更完整，线粒体各种病变发生率降低（P <0.05），提示各治疗组对COPD 大鼠有积极治疗作用。结论GBE 能够延缓和改善COPD 大鼠模型的形成。

Abstract:

Objective To study the intervention effect of Ginkgo biloba extract (GBE) on the pulmonary ultrastructural changes in rats with chronic obstructive pulmonary disease (COPD). Methods Ninety Wistar rats were randomly divided into normal control group (group A), COPD control group (group B), early-stage GBE treatment group (group C), late-stage GBE treatment group (group D), Erythromycin early-treatment group (group E), and Erythromycin late-stage treatment group (group F). Six rats were randomly seleted from each group, ultrastructural changes of the lungs were observed by transmission electron microscopy, and the rates of pathological changes of mitochondria were calculated. Results The groups B, C, D, E and F all showed characteristic pathological changes of COPD to different extent. Under the transmission electron microscope,the ultrastructures in the groups C, D, E and F were more complete than those in the group B, and the rates of mitochondrial lesions were conspicuously lowered in the treatment groups, suggesting that GBE had a positive therapeutic effect. Conclusions GBE has inhibitory effects on airway and vascular remodeling in rat model of COPD.