自发性高血压大鼠心肾交感神经的分布状况

Distribution of heart and kidney sympathetic nerves in spontaneous hypertension rats

摘要:

目的探讨自发性高血压大鼠心脏及肾脏交感神经的分布状况。方法选取6及12周龄的自发性高血压大鼠（SHR）和相应周龄的同源正常血压大鼠（WKY），以儿茶酚胺合成限速酶酪氨酸羟化酶（TH）的表达作为交感神经分布的标志，采用免疫组织化学检测TH，尾套法检测大鼠血压及心率。结果6及12周龄的SHR血压及心率高于相应周龄的WKY 鼠。6 及12 周龄的SHR心脏及肾脏TH 的表达高于相应周龄的WKY 鼠。SHR12 周龄时肾脏TH的表达高于6 周龄，心脏TH的表达在2 个周龄组差异无统计学意义。结论SHR心及肾脏交感神经纤维分布密度增加，并且随着高血压的进展，肾脏交感神经纤维分布密度有增加的趋势。

Abstract:

Objective To investigate the cardiac and nephrological distribution of sympathetic nerves in spontaneous hypertension rats (SHR). Methods Heart and liver tissues were harvested from 6-week and 12-week old SHR and homogenous Wistar-Kyoto rats (WKY). Immunohistochemistry staining of Tyrosine hydroxy- lase (TH), a catecholamine synthesis rate-limiting enzyme, was performed as an indication of sympathetic nerves.Blood pressure and heart rate were measured by tail-cuff method. Results Both blood pressure and heart rate of 6-week and 12-week old SHR were significantly increased compared with those of WKY. Expression levels of TH in heart and kidney of 6-week and 12-week old SHR were significantly higher than those of WKY. In SHR groups, TH expression in kidney of 12-week upregulated significantly when compared with that of the 6-week group, while there was no difference in terms of hepatic TH between 6-week and 12-week old ones. Conclusion The density of sympathetic nerves is much higher in SHR than that in WKY; nephrological sympa-thetic nerves were increased in time-dependent manner alongside the progression of hypertension.