结肠癌患者肿瘤微环境中T细胞亚群与肿瘤细胞转移的关系

Correlations between T cell subsets in tumor microenvironment and tumor cell metastasis in patients with colon cancer

摘要:

 摘要：目的  探讨结肠癌患者肿瘤微环境中T细胞亚群与肿瘤细胞转移的关系。方法  前瞻性收集该院收治的结肠癌患者89例，所有患者行结肠癌切除术，收集术中肿瘤标本，检测肿瘤组织中CD4+ T细胞、CD8+ T细胞和调节性T细胞。分析CD4+ T细胞、CD8+ T细胞及调节性T细胞与患者临床特征关系。结果  随着TNM分期的增加，CD4+ T细胞和调节性T细胞增加，CD8+ T细胞降低（P <0.05）。高分化、中分化及低分化患者调节性T细胞分别为（6.59±1.87）%、（7.27±1.81）%和（8.12±1.92）%，差异有统计学意义（P <0.05）。合并淋巴结转移患者CD4+ T细胞增高，CD8+ T细胞降低，调节性T细胞升高（P <0.05）。腺癌、黏液癌期未分化癌调节性T细胞分别为（6.82±1.88）%、（7.28±1.82）%和（8.22±1.91）%，差异有统计学意义（P <0.05）。结论  肿瘤微环境中T细胞亚群失衡与肿瘤细胞的转移相关，调节微环境中T细胞水平或可改善患者临床预后。

Abstract:

Abstract: Objective To investigate the relationships between T cell subsets in tumor microenvironment and tumor cell metastasis in patients with colon cancer. Methods A total of 89 patients with colon cancer in our hospital were enrolled. All patients underwent resection of colon cancer. The tumor specimens were collected. CD4+ T cells, CD8+ T cells and regulatory T cells in the tumor tissues were detected, and their correlations with clinical features of colon cancer patients were studied. Results With the increase of TNM staging, CD4+ T cells and regulatory T cells increased, and CD8+ T cells decreased (P < 0.05). The regulatory T cells of the patients with well-differentiated, moderately-differentiated and poorly-differentiated colon cancers were (6.59 ± 1.87) %, (7.27 ± 1.81) % and (8.12 ± 1.92) % respectively, and the differences were statistically significant (P < 0.05). CD4+ T cells and regulatory T cells were increased, while CD8+ T cells were decreased in the patients with lymph node metastasis (P < 0.05). The regulatory T cells of adenocarcinoma, mucinous carcinoma and undifferentiated carcinoma were (6.82 ± 1.88) %, (7.28 ± 1.82) % and (8.22 ± 1.91) % respectively, and the differences were statistically significant (P < 0.05). Conclusions The imbalance of T cell subsets in tumor microenvironment is related to the metastasis of tumor cells, therefore regulation of the T cells in the microenvironment could improve the prognosis.