水飞蓟素对大鼠肾脏缺血再灌注损伤的保护作用及机制探讨

Protective effect of Silymarin on renal ischemia-reperfusion injury in rats

摘要:

目的探索水飞蓟素对大鼠肾脏缺血再灌注损伤（IRI）的保护作用及是否与抑制NF-κB 活化有关。方法将30 只成年雄性SD 大鼠随机为假手术组（S 组）、缺血再灌注模型组（IR 组）及药物组（SM 组）。全自动生化分析仪检测血肌酐、血尿素氮，HE 染色检测肾小管损伤程度，免疫组织化学检测肾脏IL-6 及NF-κB p65 表达，酶联免疫吸附法测定肾组织匀浆上清液NF-κB、IL-6 浓度。结果IR组血肌酐、尿素氮、组织匀浆细胞核内NF-κB 及细胞浆中IL-6 水平较S 组升高（P <0.05），SM 组较IR 组降低（P <0.05），IR 组肾小管损伤较重，肾脏IL-6、NF-κB p65 表达增强。而给药组肾小管损伤减轻，肾脏IL-6、NF-κB p65 表达减弱。结论水飞蓟素对大鼠肾脏缺血再灌注损伤具有保护作用，其与抑制NF-κB活化有关。

Abstract:

Objective To investigate the protective effect of Silymarin on renal ischemia-reperfusion injury(IRI) and its underlying inhibiting mechanism towards nuclear factor kB (NF-kB). Methods A total of 30 male sprague-dawley (SD) rats were randomly divided into three groups: sham operation (S) group, IRI (IR) group and IRI plus Silymarin (SM) group. Serum creatinine (Cr) and blood urea nitrogen (BUN) were analyzed with automatic biochemical analyzer. Hematoxylin-eosin (HE) staining was performed for histological grading of kidney injury. Expression of interleukin 6 (IL-6) and NF-kB p65 was identified by Immunohistochemical staining and Enzyme-linked immunosorbent assay (ELISA). Results Circulating Cr, BUN, NF-kB and IL-6 were elevated significantly in the IR group compared with those in the S group (P < 0.05), and those in the SM group (P < 0.05). Animals in the IR group experienced obvious increase of renal tubular damage and expression of IL-6 and NF-kB, which were ameliorated with treatment of Silymarin. Conclusions Inactivation of NF-kB signaling pathway mediates protective effect of Silymarin on renal ischemia-reperfusion.