急性单核细胞白血病全基因组微小RNA的表达谱分析

Whole-genome analysis profile of MicroRNA in acute monocytic leukemia

摘要:

目的研究急性单核细胞白血病（AMoL）全基因组的微小RNA（miRNA）表达谱。方法应用Illumina高通量测序技术对10 例AMoL 患者和10 例非恶性血液病患者骨髓样本的miRNA 表达水平进行检测，分析两者的表达差异，并通过茎环引物实时荧光定量PCR（Stem-loop qPCR）技术对部分Illumina 测序结果进行验证。结果两组样本共发现差异表达的miRNA 285个，其中，199 个miRNA 在AMoL组呈表达上调，86 个miRNA呈表达下调。6 个miRNA 的Stem-loop qPCR 验证结果与其测序结果具有相同的变化趋势。结论miR-126-3p 和miR-29b-3p 可作为潜在的分子靶点用于AMoL发病机制的进一步研究。

关键词:急性单核细胞白血病;微小RNA;测序

Abstract:

Objective To investigate the whole-genome MicroRNA (miRNA) profile in acute monocytic leukemia (AMoL). Methods Illumina high -throughput sequencing technology was utilized to screen differentially expressed miRNA in bone marrow samples of 10 AMoL patients as well as 10 patients with non-malignant hematologic diseases. Stem-loop primer-based real-time quantitative polymerase chain reaction (Stem-loop qPCR) was carried out to validate the sequencing data. Results In total of 285 differentially expressed miRNAs, 199 up -regulated and 86 down -regulated miRNAs were identified in AMoL patients compared with the control group. Further stem-loop qPCR results confirmed similar expression of 6 miRNAs identified by sequencing. Conclusions MiR-126-3p and miR-29b-3p can be potential targets for further study of AMoL.

Key words:acute monocytic leukemia; micro ribonucleic acid; sequencing