长链非编码RNA-尿路上皮癌相关分子1在宫颈癌中的表达及临床意义

Significance of long non-coding RNA urothelial cancer associated 1 in cervical cancer

摘要:

目的检测长链非编码RNA（lncRNA）-尿路上皮癌相关分子（UCA1）在宫颈癌组织中的表达，探讨UCA1 与宫颈癌临床病理特征、术后生存率及预后间的关系。方法利用lncRNA 芯片筛选宫颈癌及癌旁组织中差异表达的lncRNAs；利用实时荧光定量聚合酶链反应（qRT-PCR）检测宫颈癌组织及配对癌旁组织中UCA1的表达；利用Spearman秩相关分析UCA1 与宫颈癌患者临床病理特征之间的关系；利用生存分析曲线UCA1 与宫颈癌患者术后5 年生存率的关系；利用Cox 风险比例模型分析UCA1 与宫颈癌患者预后的关系。结果UCA1 在宫颈癌表达水平高于癌旁组织；UCA1 的表达与宫颈癌FIGO 分期相关（P =0.015）；UCA1 高表达的宫颈癌患者术后5 年生存率低于低表达的患者，UCA1 表达及FIGO 分期为宫颈癌患者独立的预后指标。结论UCA1 在宫颈癌中表达升高，并与宫颈癌患者生存及预后密切相关。

Abstract:

Objective To investigate the expression level of long non-coding RNA (lncRNA) urothelial cancer associated 1 (UCA1) in cervical cancer, and evaluate its prognostic value in cervical cancer. Methods Cancer tissue as well as adjacent normal tissue from patients diagnosed with cervical cancer were harvested in this study. LncRNA arrays were performed to identify differentially expressed lncRNAs. Expression of UCA1 was measured by qRT-PCR. Spearman rank correlation analysis was performed to evaluate the association between UCA1 and clinical manifestation of cervical cancer patients. K-M survival analysis was utilized to determine correlation of UCA1 expression with 5-year survival. Cox's proportional hazards regression models were applied to analyze the relationships between UCA1 and prognosis of cervical cancer patients. Results UCA1 was upregulated in cervical cancer tissues compared with normal tissues. Expression of UCA1 was closely associated with FIGO stage of cervical cancer (P = 0.015). Expression of UCA1 was negatively correlated with 5 -years survival rate. UCA1 and FIGO stage were independent prognostic risk factor of cervical cancer. Conclusion UCA1 is upregulated in cervical cancer, and is negatively associated with outcome of cervical cancer.