miRNA-30b对抑制胃癌细胞增殖及侵袭的作用研究

Effect of miRNA-30b on cell proliferation and invasion of gastric cancer

摘要:

目的研究miRNA-30b在胃癌癌组织中的表达水平及对癌细胞增殖侵袭的影响，探讨miRNA-30b在胃癌中的临床意义及可能的分子机制。方法实时荧光定量聚合酶链反应（qRT-PCR）检测miRNA-30b在胃癌及癌旁组织中的表达情况；用miRNA-30b模拟物及抑制物转染人胃癌HGC-27细胞，CCK-8法检测细胞增殖的情况，Transwell 小室法检测细胞侵袭情况，Western blot 检测RAB22A 和USP47 蛋白表达。结果miRNA-30b在胃癌组织中表达水平低于对应癌旁组织（P <0.05），且miRNA-30b 低表达与胃癌分期及肿瘤大小有关（P <0.05）；与转染阴性对照细胞比较，转染miRNA-30b模拟物的HGC-27细胞增殖水平和侵袭能力均受到抑制（P <0.05），RAB22A和USP47 蛋白表达水平降低（P <0.05）；而转染miRNA-30b 抑制物的HGC-27细胞增殖水平和侵袭能力则增强（P <0.05），RAB22A 和USP47 蛋白表达水平升高（P <0.05）。结论miRNA-30b 在胃癌组织中表达下调，且miRNA-30b 可能通过调节RAB22A 和USP47 的表达从而影响胃癌细胞的增殖及侵袭。

Abstract:

Objective To investigate the effect of miR-30b on cell proliferation and invasion in gastric cancer and possible molecular mechanism. Methods qRT-PCR was utilized to detect expression of miR-30b in gastric cancer and adjacent tissues. HGC -27 cell line was transfected with miRNA -30b mimics or inhibitor. Cell proliferation and cell invasion was determined by CCK -8 assay and Transwell assay, respectively. RAB22A and USP47 were measured by Western blot. Results The expression of miRNA-30b in gastric cancer tissues was significantly decreased compared with adjacent tissues (P < 0.05), and low expression of miRNA-30b was closely associated with stage as well as size of tumor (P < 0.05). HGC-27 cell proliferation and invasion were significantly decreased by miRNA-30b mimics transfection compared with control group (P <0.05), which was attenuated by treatment of miRNA-30b inhibitor (P < 0.05). Expression levels of RAB22A and USP47 were decreased after miRNA-30b mimics transfection compared with control group (P < 0.05),which was attenuated by treatment of miRNA -30b inhibitor (P < 0.05). Conclusions The expression of miRNA-30b in gastric cancer is decreased, and miRNA-30b may inhibit the cell proliferation and invasion of the cancer cells through manipulation of RAB22A and USP47 pathway.