食管癌患者miR-182表达水平及其对预后的影响

Expression of miRNA-182 and its effect on prognosis of esophageal carcinoma

摘要:

目的探讨miR-182在食管癌患者组织中的基因表达及其对预后的影响。方法采用实时荧光定量PCR 检测59 例食管癌标本和癌旁组织中miR-182 表达水平；并分析其表达水平与患者的临床病理特征和随访结局之间的关系。结果相较于癌旁组织，食管癌组织中miR-182 表达水平增高（P <0.05）；miR-182 表达水平与肿瘤分化程度、浸润深度、是否发生转移以及临床分期相关（P <0.05），与性别、年龄以及肿瘤大小无关系（P >0.05）；生存分析显示miR-182 高表达组总体生存期低于低表达组（P <0.05）；Cox 多因素回归分析结果显示miR-182 表达水平可作为食管癌患者预后不良的标志之一。结论miR-182 在食管癌组织中高表达，其高表达提示预后较差，可作为食管癌潜在的预测分子标志物。

Abstract:

Objective To investigate the concentration of miRNA-182 in esophageal carcinoma and its association with disease prognosis. Methods MicroRNA-182 in 59 esophageal carcinoma tissues and related adjacent healthy tissues were measured by quantitative real time polymerase chain reaction (qRT -PCT). Association of miRNA -182 with clinical characters including pathologic types, and clinical outcome was determined. Results The concentration of miRNA -182 in esophageal carcinoma tissues was significantly increased when compared with that in adjacent healthy tissues (P < 0.05). Overexpression of miRNA-182 correlated closely with tumor differentiation level, depth of infiltration, metastasis, and clinical stage (P < 0.05). Kaplan -Meier survival analysis indicated that patients with high miRNA -182 experienced worse outcome compared with others (P < 0.05). Moreover, multivariate analysis showed that increase of miRNA-182 was an independent predictor of overall survival (P < 0.05). Conclusions MicroRNA-182 is overexpressed in esophageal carcinoma tissue and concentration of miRNA-182 is closely correlated with poor outcome, suggesting that miRNA-182 may serve as a prognostic biomarker for esophageal carcinoma.