核苷酸结合寡聚化结构域1和2基因在幽门螺杆菌相关胃癌中的表达及意义

Expression and significance of nucleotide binding oligomerization domains 1 and 2 in Helicobacter pylori-associated gastric cancer

摘要:

目的探讨核苷酸结合寡聚化结构域（NOD）1 和2基因在幽门螺杆菌（Hp）相关胃癌患者中的表达变化及意义。方法采用免疫组织化学法检测胃癌患者癌组织中NOD1和NOD2蛋白的表达；采用实时聚合酶链反应（real-time PCR）检测胃癌组织中NOD1 和NOD2 mRNA 的表达；采用Western blot检测胃癌组织中凋亡蛋白核转录因子资B（NF-资B）和半胱氨酸的天冬氨酸蛋白水解酶3（Caspase-3）表达。结果与Hp阴性胃癌患者相比，Hp阳性胃癌患者癌组织中的NOD1和NOD2 蛋白表达含量和mRNA 表达含量上升；Hp 阳性胃癌患者中的NF-κB和Caspase-3 蛋白含量升高（p <0.05）。结论Hp 阳性胃癌患者中的NOD1 和NOD2 呈高表达状态；且过表达NOD1 和NOD2 可能在Hp 阳性胃癌发生、发展机制中起重要的作用。

Abstract:

Objective To investigate the expression and significance of NOD1 and NOD2 in patients with helicobacter pylori(Hp ) associated gastric cancer. Methods Immunohistochemical method was used to detect the expression of NOD1 and NOD2 proteins in patients with Hp-associated gastric cancer. The expression of NOD1 and NOD2 mRNA was detected by real-time PCR. Western blot was used to detect the expression of NF-kB and Caspase-3. Results Compared with the control group (patients with Hp-negative gastric cancer),the expression of NOD1 and NOD2 protein and mRNA in the patients with Hp-positive gastric cancer in-creased significantly, the levels of NF-kB and Caspase-3 in the Hp-positive gastric cancer patients were in-creased (p < 0.05). Conclusions Compared with the Hp-negative gastric cancer, NOD1 and NOD2 are highly expressed in the patients with Hp-associated gastric cancer. And NOD1 and NOD2 over-expressions may play important roles in the development and progression of Hp-associated gastric cancer.