诱导型一氧化氮合酶和缺氧诱导因子-1α与尖锐湿疣发生、发展的相关性研究

Effect of iNOS and HIF-1α on condyloma acuminatum

摘要:

目的检测尖锐湿疣（CA）组织中诱导型一氧化氮合酶（iNOS）与缺氧诱导因子-1α（HIF-1α）的表达，以明确其在CA 发生、发展中的临床意义。方法收集50 例CA和20 例正常包皮组织，采用免疫组织化学法检测组织中iNOS 与HIF-1α的表达并与CD34 表达进行相关分析。结果50 例CA 患者中，iNOS 阳性50 例（100%），表现为表皮全层细胞均为iNOS 阳性表达；对照组20 例正常皮肤中iNOS 阳性14 例（70%），iNOS阳性表达主要位于表皮基底层，且呈弱阳性表达，CA 患者皮损中iNOS 灰度值为（97.985±20.320）高于对照组（73.017±15.633）（P <0.05）。50 例CA 患者中42 例HIF-1α阳性（84%），高于对照组阳性9 例（45%）（P <0.05）；且HIF-1α在CA患者中灰度值为（0.204±0.064），高于对照组（0.135±0.019）（P <0.05）。CA患者组织中iNOS 与CD34 表达呈正相关（r =0.375，p =0.007）；CA 患者组织中HIF-1α与CD34 表达呈正相关（r =0.393，p=0.005）。结论iNOS 与HIF-1α 可能协同参与CA 的发病和发展。

Abstract:

Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and hypoxia inducible factor-1α(HIF-1α) and their effect on development of condyloma acuminatum (CA). Methods A total of 50 patients with CA and 20 healthy volunteers were collected. The expression of iNOS, HIF-1α and CD34 was determined by Immunohistochemistry. Results A total of 50 cases with CA were identified as iNOS positive (100%) which were expressed in whole epidermis; 14 out of 20 volunteers (70%) were iNOS positive (weak signal) which were mainly expressed in epidermal basal cells. Gray value of iNOS in CA patients was higher than that in healthy volunteers (97.9854 ±20.3196 vs 73.0174 ±15.6330, respectively) (p < 0.05). Fortytwo out of 50 patients (84%) experienced increased expression of HIF-1α while only 9 volunteers had positive signals (45%) (p < 0.05). Gray value of HIF-1α in CA patients was higher than that in the control group (0.2035 ±0.0637 vs 0.1349 ±0.0194) (p < 0.05). Intimate correlation between iNOS or HIF-1α and CD34 in CA was observed ( r= 0.375 and 0.393,p < 0.05). Conclusion iNOS and HIF-1α is involved in the pathogenesis of CA.