口腔黏膜白斑和口腔鳞癌中Bcl-2、CDK2AP 1的基因表达和临床意义

Expression and clinical value of Bcl-2 and CDK2AP1 in oral leukoplakia and oral squamous carcinoma tissue

摘要:

目的研究在口腔黏膜白斑和口腔鳞癌组织中Bcl-2和CDK2AP1 的表达情况及临床意义。方法运用免疫组织化学和实时荧光定量PCR技术对组织中Bcl-2、CDK2AP1 表达进行检测。结果在口腔正常黏膜组织、口腔黏膜白斑组织和口腔鳞癌组织中Bcl-2 蛋白细胞阳性率依次为（2.72±1.45）%、（19.29±8.18）%和（57.20±19.37）%；CDK2AP1 蛋白细胞阳性率依次为（62.68±11.07）%、（25.68±8.17）%和（7.87±2.18）%。Bcl-2和CDK2AP1 mRNA表达水平与蛋白表达水平一致。各组之间差异有统计学意义（P <0.05）。结论随着口腔病变恶性程度增高，在口腔正常黏膜、口腔黏膜白斑和口腔鳞癌中Bcl-2 表达水平上升，而CDK2AP1 表达水平降低，这2 个指标可能与口腔肿瘤的发生、发展及预后有关。

Abstract:

Objective To investigate expression levels of Bcl-2 and CDK2AP1 in oral leukoplakia and oral squamous carcinoma tissue and its potential clinical value. Methods Patient samples of oral leukoplakia, oral squamous carcinoma and normal tissue were collected in this study. Immunohistochemistry and the fluorescence quantitative PCR were utilized to identify expression of Bcl -2 and CDK2AP1. Results Immunohistochemistry data suggested that ratio of Bcl-2 positive cells in oral leukoplakia and oral squamous cell carcinoma tissue were increased significantly when compared with normal oral mucosa [(19.29 ±8.18)% vs(2.72 ±1.45) % (P < 0.05), (57.20 ±19.37)% vs (2.72 ±1.45) % ( P< 0.05), respectively]. However, ratio of CDK2AP1 positive cells in oral leukoplakia and oral squamous cell carcinoma tissue were decreased dramatically when compared with normal oral mucosa [(25.68 ±8.17)% vs (62.68 ±11.07) % ( P< 0.05), (7.87±2.18)% vs (62.68 ±11.07) % ( P< 0.05), respectively]. Immunohistochemistry data were well echoed by PCR results and statistical significance among each groups were obtained. Conclusion Expression levels of Bcl-2 increase while CDK2AP1 decreases along with the increase of malignancy from normal oral mucosa, oral leukoplakia to oral squamous cell carcinoma tissue, which may indicates that Bcl-2 and CDK2AP1 may be involved in development as well as prognosis of oral tumors.