循环微粒对口腔癌进展的作用机制研究

Role of CMPs in development of oral squamous cell carcinoma

摘要:

目的比较正常人和口腔鳞状细胞癌患者循环微粒的含量，并探究循环微粒在口腔癌进展中的作用。方法差速离心法收集正常人和口腔癌循环微粒。流式细胞术检测循环微粒的浓度；MTT 法和EdU 实验检测细胞增殖活性，划痕实验和Transwell 实验分别检测细胞迁移和侵袭能力；裸鼠移植瘤模型检测循环微粒对肿瘤细胞体内成瘤的影响。结果口腔癌患者循环微粒的含量比正常人的高，且循环微粒的浓度与口腔癌患者血液高凝状态密切相关。口腔癌患者来源的循环微粒促进口腔癌细胞增殖、迁移、侵袭和体内成瘤。结论口腔癌患者来源的循环微粒促进口腔癌细胞增殖、迁移、侵袭和体内成瘤，在口腔癌的发生、发展中起重要作用。

Abstract:

Objective To evaluate the role of circulating microparticles (CMPs) in the development of oral squamous cell carcinoma (OSCC). Methods CMPs were isolated by gradient centrifuge from healthy volunteers and OSCC patients. Cell proliferation was determined by MTT assay and EdU test. Migration and invasion activity were evaluated by wound healing assay and Transwell, respectively. Nude mouse models were utilized to evaluate tumorigenesis capacity of OSCC xenograft with or without CMPs. Results The concentration of CMPs was significantly higher in OSCC patients compared with healthy volunteers, and the increase of CMPs was closely associated with hyper -coagulation status. The ability of proliferation, migration, invasion and tumorigenesis of CAL27 cells were significantly enhanced by OSCC-derived CMPs. Conclusions OSCC-derived CMPs can increase proliferation, migration, invasion and tumorigenesis of CAL27 cells.