下调 α-catulin 基因表达对胃癌细胞增殖、迁移和侵袭的影响

Genetic inhibition of α-catulin on proliferation, migration and invasion of gastric cancer cells

摘要:

目的探讨抑制α-catulin基因表达对胃癌细胞增殖、迁移和侵袭的影响，以及可能的机制。方法培养人胃癌细胞SGC-7901，根据转染物不同将细胞分为siRNA-α-catulin组、siRNA- 对照序列组和空白对照组，MTT 法检测细胞增殖能力，Transwell 法检测细胞迁移和侵袭能力，Western blot检测细胞中α-catulin、N- 钙粘蛋白（N-cadherin）、E- 钙粘蛋白（E-cadherin）和波形蛋白（Vimentin）表达。结果siRNA-α-catulin组细胞24、48、72及96 h时吸光度A值均低于siRNA-对照序列组和空白对照组，差异有统计学意义（P <0.05）；与siRNA-对照序列组和空白对照组比较，siRNA-α-catulin组迁移细胞数和侵袭细胞数均降低，差异有统计学意义（P <0.05）；与siRNA- 对照序列组和空白对照组比较，siRNA-α-catulin 组细胞中α-catulin、N-cadherin及Vimentin 蛋白相对表达量均降低，而E-cadherin 蛋白相对表达量增加，差异有统计学意义（P <0.05）。结论特异性沉默人胃癌细胞SGC-7901 中基因表达可有效抑制细胞增殖、迁移和侵袭能力，其机制可能与抑制上皮- 间质转化过程有关。

Abstract:

Objective To investigate the effect of genetic inhibition of on proliferation, migration and invasion of gastric cancer cells. Methods Human gastric cancer cell line SGC-7901 were cultured and were divided into siRNA-α-catulin group, siRNA sequence control group and negative control group. MTT and Transwell assay were performed to measure cell proliferation and cell invasion, respectively. The levels of α-catulin, N-cadherin, E-cadherin and Vimentin were determined by Western blot. Results MTT results indicated that optical density value at 24 h, 48 h, 72 h and 96 h in the siRNA-α-catulin group were significantly lower than that of siRNA-control sequence group and negative control group (P < 0.05). The amount of migrating cells and invasive cells in siRNA-α-catulin group were decreased dramatically compared with siRNA-control sequence group and blank control group (P < 0.05). Expression levels of α-catulin, Ncadherin and Vimentin in siRNA-α-catulin group decreased while E-cadherin increased significantly when compared with siRNA -control sequence group and blank control group (P < 0.05). Conclusion Genetic knockdown of gene in SGC-7901 cell line could prevent cell proliferation, migration and invasion by inhibition of transition of epithelial-mesenchymal.