乳鼠雪旺细胞对自体颅骨组织生长的影响

Effect of Schwann cells on growth of autogenous skull in neonatal rats

摘要:

目的 探讨乳鼠雪旺细胞对自体颅骨组织生长的影响。方法 体外培养和纯化乳鼠雪旺细胞，取乳鼠颅骨组织分为对照组和观察组。对照组颅骨组织单独培养，观察组颅骨组织与雪旺细胞共同培养，培养第1、2和3周时免疫组织化学法测定颅骨组织骨钙素和骨桥蛋白水平，酶联免疫法吸附法测定培养液中骨碱性磷酸酶活性含量。结果 雪旺细胞在体外被成功培养，纯度>89%。不同时间点骨钙素水平有差别（P <0.05）；观察组和对照组骨钙素水平有差别（P <0.05），观察组骨钙素水平比对照组高；观察组和对照组骨钙素水平变化趋势有差别（P <0.05）。不同时间点骨桥蛋白水平无差别（P >0.05）；观察组和对照组骨桥蛋白水平无差别（P >0.05）；观察组和对照组骨桥蛋白水平变化趋势无差别（P >0.05）。不同时间点骨碱性磷酸酶活性有差别（P <0.05）；观察组和对照组骨碱性磷酸酶活性有差别（P <0.05），观察组骨碱性磷酸酶活性比对照组高；观察组和对照组骨碱性磷酸酶活性变化趋势有差别（P <0.05）。结论 雪旺细胞可以提高颅骨组织骨钙素水平和骨碱性磷酸酶活性，促进颅骨组织的生长。

Abstract:

Objective To investigate the effect of Schwann cells on the growth characteristics of autogenous skull tissue in neonatal SD rats. Methods The neonatal Schwann cells were cultured and purified in vitro. The skull tissues were divided into control group and observation group. The skull tissue of the control group was cultured alone. The skull tissue of the observation group was co-cultured with Schwann cells. In the 1st, 2nd and 3rd weeks of culture, the levels of osteocalcin and osteopontin in the skull tissue were measured by immunohistochemistry, and the bone alkaline phosphatase activity was measured by ELISA. Results Schwann cells were successfully cultured in vitro with the purity over 89%. There were differences in the osteocalcin levels at different time points (P < 0.05). There were significant differences in the osteocalcin levels between the observation group and the control group (P < 0.05), the levels of osteocalcin in the observation group were higher than those in the control group. There was significant difference in the change trends of osteocalcin levels between the observation group and the control group (P < 0.05). There were no significant differences in the osteopontin levels at different time points (P > 0.05). There were no significant differences in the osteopontin levels between the observation group and the control group (P > 0.05). There was no significant difference in the change trends of osteopontin levels between the observation group and the control group (P > 0.05). The activity of bone alkaline phosphatase was different at different time points (P < 0.05). There was significant difference in the bone alkaline phosphatase activity between the observation group and the control group (P < 0.05), the activity of bone alkaline phosphatase in the observation group was higher than that in the control group. There was significant difference in the change trends of bone alkaline phosphatase activity between the observation group and the control group (P < 0.05). Conclusions Schwann cells can increase osteocalcin level and bone alkaline phosphatase activity of skull tissue, and promote the growth of skull tissue.