HOX 转录反义RNA 表达对胸腔镜食管癌切除术后风险的预测价值研究

HOX Transcription Antisense RNA as prognostic biomarker in Patients with Thoracoscopic Esophageal Carcinoma

摘要:

目的 探讨HOX 转录反义RNA 表达对胸腔镜食管癌切除术后风险的预测价值。方法 收集该院接受胸腔镜食管癌切除术患者92 例，采用RT-PCR 检测术后组织中HOX 转录反义RNA 表达情况，分析HOX 转录反义RNA 表达与食管癌术后复发、生存状况之间的关系。结果 RT-PCR 显示，癌组织HOTAIR 相对表达量为（0.673±0.076），而癌旁组织为（0.387±0.058），两组比较差异有统计学意义（P <0.05）。HOTAIR 对术后复发诊断的曲线下面积为0.727（95%CI ：0.684，0.884），对术后生存诊断的曲线下面积为0.769（95%CI ：0.692，0.901）。术后3 年内，HOTAIR 相对表达量≤ 0.5 组复发率低于HOTAIR 相对表达量>0.5 组（P <0.05）。Cox 模型分析显示，分化程度、临床分期、肿瘤直径、淋巴结转移及HOTAIR 为预后的危险因素，而术后辅助放、化疗为预后的保护因素。结论 HOTAIR 为胸腔镜食管癌切除术后复发、生存状况的影响因素，通过对HOTAIR 的检测可以为临床选择合理的治疗方案提供参考依据。

Abstract:

Objective To investigate the prognostic value of HOX transcribed antisense RNA expression in patients with thoracoscopic esophagostomy. Methods Totally 92 patients undergoing thoracoscopic esophageal cancer resection were included in this study. Cancer tissue as well as para-cancer normal tissue was collected.RT-PCR was utilized to detect the expression of HOX transcribed antisense RNA. Correlation between HOX transcribed antisense RNA expression and postoperative recurrence as well as survival was analyzed. Results RTPCR showed that the relative expression of HOTAIR in cancer tissue increased significantly when compared with that in normal tissue [(0.673 ± 0.076) vs (0.387 ± 0.058), P < 0.05]. The area under the curve of HOTAIR for postoperative recurrence was 0.727 (95% CI: 0.684, 0.884), and that for postoperative survival was 0.769 (95% CI:0.692, 0.901). At time point of the 3rd year post operation, recurrence rate in group with HOTAIR less than 0.5 was dramatically lower than that of group with HOTAIR > 0.5 group (P < 0.05). Cox model analysis indicated that the degree of differentiation, clinical stage, tumor diameter, lymph node metastasis and HOTAIR were risk factors while postoperative adjuvant radiotherapy and chemotherapy was beneficial for the prognosis of patients. Conclusion HOTAIR is the risk factor for the prognosis of patients experiencing thoracoscopic esophagostomy, which can serve as a prognostic biomarker of esophageal cancer.