狼疮性肾炎患者血清IL-18、IL-34 水平及其意义

Levels of serum IL-18 and IL-34 in patients with lupus nephritis and their significance

摘要:

目的 探讨狼疮性肾炎患者血清白细胞介素18（IL-18）和白细胞介素34（IL-34）水平及其意义。方法 收集2013 年1 月-2016 年12 月吉林大学第一医院狼疮性肾炎患者70 例作为狼疮性肾炎组，健康体检者70 例作为对照组；狼疮性肾炎组患者根据系统性红斑狼疮疾病活动度评分（SLEDAI）-2000 分为活动组（SLEDAI 评分≥ 10 分）45 例和非活动组（SLEDAI 评分<10 分）25 例。收集两组患者临床资料，采用酶联免疫吸附法测定血清IL-18 和IL-34 水平。结果 狼疮性肾炎组血清IL-18 和IL-34 水平高于对照组（P <0.05）。狼疮性肾炎活动组血清IL-18 和IL-34 水平高于非活动组（P <0.05）。狼疮性肾炎患者血清IL-18 和IL-34水平与C 反应蛋白、24 h 尿蛋白、ds-DNA、SLEDAI 评分呈正相关（P <0.05），狼疮性肾炎患者血清IL-34水平与补体C3 呈负相关（P <0.05）。狼疮性肾炎患者血清IL-18 水平与肾小管上皮细胞变性、肾小管白细胞管型、肾小管炎症细胞浸润，肾小球增生性改变、肾小球炎症细胞浸润，肾间质纤维化呈正相关（P <0.05）。狼疮性肾炎患者血清IL-34 水平与肾小管上皮细胞变性、肾小管白细胞管型、肾小管炎症细胞浸润，肾小球增生性改变、肾小球炎症细胞浸润，肾间质纤维化、肾间质炎症细胞浸润呈正相关（P <0.05）。结论 狼疮性肾炎患者血清IL-18 和IL-34 水平升高，血清IL-18、IL-34 水平与疾病活动性及病理变化呈正相关。

Abstract:

Objective To investigate the levels of serum interleukin 18 (IL-18) and interleukin 34 (IL-34) in patients with lupus nephritis and their significance. Methods Seventy cases of patients with lupus nephritis were collected as lupus nephritis group, and 70 healthy subjects were collected as control group in the First Hospital of Jilin University from January 2013 to December 2016. The patients with lupus nephritis were divided into active group(SLEDAI ≥ 10) and inactive group (SLEDAI <10) according to Systemic Lupus Erythematosus Disease Activity Score (SLEDAI) -2000 score, each group had 25 cases. The clinical data of the two groups were collected. The serum IL-18 and IL-34 levels were measured by ELISA. Results The levels of serum IL-18 and IL-34 in the lupus nephritis group were higher than those in the control group (P < 0.05). The levels of serum IL-18 and IL-34 in the active group were higher than those in the inactive group (P < 0.05). The levels of serum IL-18 and IL-34 in the patients with lupus nephritis were positively correlated with C-reactive protein, 24-h urinary protein, ds-DNA and SLEDAI score (P < 0.05). There was a negative correlation between serum IL-34 level and complement C3 level in the patients with lupus nephritis (P < 0.05). The serum IL-18 level was positively correlated with renal tubular epithelial cell degeneration, renal tubular leukocyte casts, inflammatory cell infiltration in renal tubules, glomerular hyperplasia, glomerular inflammatory cell infiltration and renal interstitial fibrosis in the patients with lupus nephritis (P < 0.05).The serum IL-34 level in the patients with lupus nephritis was positively correlated with renal tubular epithelial cell degeneration, leukocyte casts, renal tubular inflammatory cell infiltration, glomerular hyperplasia, glomerular inflammatory cell infiltration, renal interstitial fibrosis and inflammatory cell infiltration (P < 0.05). Conclusions The serum IL-18 and IL-34 levels are elevated in the patients with lupus nephritis, and in positive correlation with disease activity and pathological changes.