宫颈癌组织中CDK8、p53、VEGF 表达水平与肿瘤血管生成的关系研究

Expressions of CDK8, p53 and VEGF in cervical cancer and their correlations with angiogenesis

摘要:

目的 探讨宫颈癌组织中细胞周期依赖性蛋白激酶8（CDK8）、p53、血管内皮生长因子（VEGF）表达水平，及其与肿瘤血管生成的关系。方法 收集该院收治的宫颈癌患者60 例，检测宫颈癌组织中CDK8、p53、VEGF 及微血管密度（MVD），分析CDK8、p53、VEGF、MVD 与宫颈癌患者主要临床特征的关系。结果 VEGF 阳性患者MVD 水平高于VEGF 阴性患者（P <0.05），CDK8 阳性患者MVD 水平高于CDK8 阴性的患者（P <0.05）。p53 阳性与阴性患者MVD 水平比较，差异无统计学意义（P >0.05）。与癌旁组织比较，宫颈癌组织中VEGF（+）、CDK8（+）、p53（+）及MVD 水平升高（P <0.05）。与宫颈癌国际妇产科联盟分期Ⅰ期患者相比，Ⅱ～Ⅳ期患者的VEGF（+）、CDK8（+）、p53（+）及MVD 水平升高（P <0.05）。与肿瘤细胞高分化患者相比，中、低分化患者的CDK8（+）水平升高（P <0.05）。与鳞癌患者相比，腺癌患者的VEGF（+）和MVD 水平升高（P <0.05）。多元线性回归分析显示，VEGF 和CDK8 是宫颈癌组织中MVD 水平的影响因素（P <0.05）。结论 宫颈癌组织中CDK8、VEGF 和p53 蛋白表达水平升高，促进肿瘤细胞的生长和转移，CDK8、VEGF 可能与肿瘤血管生成有关。

Abstract:

Objective To investigate the expressions of cyclin-dependent kinase 8 (CDK8), p53 and vascular endothelial growth factor (VEGF) in the cervical cancer tissues and their correlations with tumor angiogenesis. Methods Sixty cervical cancer patients were collected in our hospital. And the CDK8, p53, VEGF and microvessel density (MVD) in the cervical cancer tissues were detected. The correlations of CDK8, p53, VEGF and MVD with the main clinical features of cervical cancer were studied. Results MVD of the VEGF-positive patients was higher than that of the VEGF-negative patients (P < 0.05); MVD of the CDK8-positive patients was higher than that of the CDK8-negative patients (P < 0.05); there was no significant difference in MVD between the patients with negative p53 and those with positive p53 (P > 0.05). Compared with the adjacent tissues, VEGF(+), CDK8(+) and p53(+) rates and MVD were significantly increased in the cervical cancer tissues (P < 0.05). Compared with the patients of International Federation of Gynecology and Obstetrics (FIGO) stage I, the patients of FIGO stage II-IV got increased VEGF(+), CDK8(+) and p53(+) rates and MVD (P < 0.05). When compared with the patients with highly-differentiated carcinoma, the patients with moderately- or poorly-differentiated carcinoma got a higher CDK8(+) rate (P < 0.05). Compared with the squamous carcinoma patients, the patients with adenocarcinoma got a higher VEGF(+) rate and increased MVD (P < 0.05). Multiple linear regression analysis showed that VEGF and CDK8 were the factors influencing MVD in the cervical cancer tissues (P < 0.05). Conclusions The expression levels of CDK8, VEGF and p53 are elevated in cervical cancer tissues, which can promote the growth and metastasis of tumor cells. And CDK8 and VEGF may be related to tumor angiogenesis.