口腔种植体周围炎与炎症细胞因子的相关性研究

Relationships between oral peri-implantitis and inflammatory cytokines

摘要:

目的 探讨口腔种植体周围炎与周围龈沟液中炎症因子水平的相关性。方法 选取2014 年4 月-2016 年4 月在该院口腔科行种植体修复的120 例患者为研究对象。种植体数目128 颗，根据种植体周围是否有炎症分为健康种植体组（81 颗）和炎症种植体组（47颗），把对侧同名天然健康牙作为对照组（62 颗）。收集并称量龈沟液重量，进行牙周学检测，包括菌斑指数（PLI）、出血指数（SBI）和探诊深度（PD）；酶联免疫吸附法检测龈沟液中白细胞介素6（IL-6）、白细胞介素1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和基质金属蛋白酶-8（MMP-8）的浓度，分析PLI、SBI、PD 值与龈沟液中IL-6、IL-1β、TNF-α、MMP-8 水平的相关性。结果 炎症种植体组PLI、SBI、PD 及龈沟液中炎症因子水平高于健康种植体组和对照组（P <0.05）；健康种植体组与对照组比较差异无统计学意义（P >05）。SBI、PLI、PD 与炎症因子水平呈正相关（P <0.05）。结论 种植体周围炎与周围龈沟液中炎症因子密切相关，炎症因子可在一定程度上反映种植体周围组织的健康状态。

Abstract:

Objective To investigate the relationships between the peri-implantitis of the oral cavity and the levels of inflammatory factors in the surrounding gingival crevicular fluid (GCF). Methods One hundred and twenty patients with implant restoration from April 2014 to April 2016 were enrolled in this study. There were totally 128 implants , which were divided into healthy implant group (81) and inflammatory implant group (47), while the corresponding contralateral natural healthy teeth were used as the control group (62). GCF was collected, and the periodontal examination was performed which included plaque index (PLI), probing depth (PD) and sulcus bleeding index (SBI). The levels of IL-6, IL-1β, TNF-α and MMP-8 in the GCF were measured by ELISA. The correlations of PLI, SBI and PD with the IL-6, IL-1β, TNF-α and MMP-8 levels in the GCF were analyzed. Results The levels of IL-6, IL-1β, TNF-α and MMP-8 in the GCF, and PLI, SBI and PD in the inflammatory implant group were higher than those in the healthy implant group and the control group, the differences were statistically significant (P < 0.05); while there were no significant differences between the healthy implant group and the control group (P > 0.05). SBI,PLI and PD were positively correlated with IL-6, IL-1β, TNF-α and MMP-8 levels (P < 0.05). Conclusions Periimplantitis is closely related to the inflammatory factors in the gingival crevicular fluid, and the inflammatory factors could reflect the health status of the peri-implant tissues to a certain extent.