氧自由基在肝缺血再灌注损伤中的作用及凯西莱的影响

ROLE OF OXYGEN FREE RADICAL AND INFLUENCE OF TIOPRONIN IN HEPATIC ISCHEMIA AND REPERFUSION INJURY

摘要:

目的 :探讨氧自由基在肝缺血再灌注损伤 (HIRI)中的作用及凯西莱的防护作用。方法 :应用HIRI家兔 ,观察超氧化物歧化酶 (SOD)、丙二醛 (MDA)、谷丙转氨酶 (ALT)和谷草转氨酶 (AST)含量变化、肝细胞形态变化及凯西莱对上述指标的影响。结果 :HIRI时 ,SOD含量降低 (P <0 .0 1) ,MDA含量增加 (P <0 .0 1) ,ALT及AST活性升高 (P <0 .0 1) ;肝细胞形态学发生异常变化 ;应用凯西莱后 ,上述指标的异常变化均明显减轻(P <0 .0 1)。结论 :氧自由基是HIRI的重要发病因素 ,凯西莱通过清除氧自由基可减轻HIRI。

Abstract:

Objective:To evaluate the role of oxygen free radicals in hepatic ischemia and reperfusion injury(HIRI) and the influence of tiopronin.Methods:Using HIRI model in rabbits, the contents of SOD,MDA, ALT and AST were measured. The changes of hepatic histology were also examined. The role of prevention and treatment of tiopronin on HIRI was also observed.Results:The contents of SOD were significantly decreased( P <0.01), whereas the levels of MDA, ALT and AST were markedly increased( P <0.01) during HIRI. The morphological changes of hepatocytes were obvious after HIRI. After prevention and treatment with tiopronin, the variations of all the parameters were alleriated. Conclusion:Oxygen free radicals might participate in injury of HIRI. Tiopronin might alleviate the injury of HIRI by eliminating oxygen free radicals.