海带多糖对超重环境下微波辐射损伤的防治

Prevention and treatment effects of laminarin on overoxidation damage of rats induced by microwave radiation under supergravity

摘要:

目的 探讨海带多糖对超重环境下微波辐射损伤的防治作用。方法 制作大鼠超重环境下微波损伤模型，通过灌胃给予20 mg/ml海带多糖（低剂量组）和40 mg/ml海带多糖（高剂量组），观察防护效果。结果 血浆中超氧阴离子在模型组显著低于对照组（P <0.05），低剂量海带多糖组和高剂量组与对照组比较差异无统计学意义（P >0.05）。丙二醛（MDA）和超氧化物歧化酶（SOD）在模型组与对照组差异无统计学意义（P >0.05），高剂量组及低剂量组显著高于对照组（P <0.05）。过氧化氢酶（CAT）在模型组显著低于对照组（P <0.05），而低剂量组与对照组比较差异无统计学意义（P >0.05）。谷胱甘肽过氧化物酶（GSH-POX）在模型组与对照组差异有统计学意义（P <0.05）；高剂量组与对照组比较差异无统计学意义（P <0.05）。一氧化碳（NO）、一氧化碳合酶（NOS）和还原型合胱甘肽（GSH）在模型组、低剂量海带多糖组和高剂量组与对照组比较差异有统计学意义（P <0.05）。在肝组织匀浆中，过氧化氢酶（CAT）在模型组降低，但与对照组比较差异无统计学意义（P >0.05）；在低剂量组与对照组比较差异无统计学意义（P >0.05）。NOS在模型组显著高于对照组（P <0.05），但低剂量组和高剂量组与对照组比较差异无统计学意义（P >0.05）。GSH-POX在模型组显著低于对照组（P <0.05）。GSH在模型组显著高于对照组（P <0.05），低剂量组与对照组比较差异无统计学意义（P >0.05）。结论 海带对超重微波的过氧化损伤的防护的调节可能通过CAT及GSH-POX的清除和增强还原型GSH的抗氧化来实现，具体机制有待进一步研究。

Abstract:

【Objective】 To research prevention and treatment effects of laminarin on overoxidation damage of microwave radiation in rats under supergravity. 【Methods】 The rat model of microwave damage under supergravity was made. The rats were given 20 mg/ml (lower-dose group) or 40 mg/ml laminarin (high-dose group) by intragastric administration and the protective effects were observed. 【Results】 The plasma superoxide anion was significantly lower in the model group than in the control group (P < 0.05), but not significantly different between either the lower-dose or high-dose group and the control group (P > 0.05). MDA and SOD were not obviously different between the model group and control group (P > 0.05). MDA and SOD in the lower-dose and high-dose groups were significantly higher than those in the control group (P < 0.05). Catalase (CAT) decreased significantly in the model group compared with the control group (P < 0.05). GSH-POX had a significant difference between the model group and control group (P < 0.05), but no significant difference between the high-dose group and the control group (P > 0.05). NO, NOS and GSH in the model group, lower-dose group and high-dose group were significantly different from those in the control group (P < 0.05). In liver homogenate CAT decreased in the model group, but had no obvious difference from that in the control group(P > 0.05). GSH-POX was significantly lower in the model group than that in the control group (P < 0.05). GSH was significantly higher in the model group than in the control group (P < 0.05), but was not significantly different between the lower-dose group and the control group (P > 0.05). 【Conclusions】 Laminarin regulation to overoxidation damage of microwave radiation under supergravity could clear reactive oxygen species by CAT and GSH-POX and increase GSH antioxidation power. The concrete mechanism needs further study.