采用16S rRNA基因分析技术鉴定正畸患者矫治前龈沟液的细菌种类

Identification of bacterial species in gingival crevicular fluid of malocclusion orthodontic patients before correction by 16S rRNA gene sequencing

摘要:

目的  了解安氏Ⅱ类错■畸形患者矫治前期龈沟液的细菌分布情况，为进一步探讨细菌感染对安氏Ⅱ类错■畸形矫治后牙根吸收的影响奠定基础。方法  从中南大学口腔医学院收集24例安氏Ⅱ类错■畸形的12~18岁患者龈沟液，在厌氧条件下进行细菌培养，PCR扩增细菌16S rRNA基因序列，经测序后通过序列比对鉴定细菌菌株。结果  共鉴定出43株菌，其中链球菌属18种菌株，奈瑟菌属7种菌株，放线菌属6种菌株，孪生球菌属2种菌株，罗氏菌属2种菌株，艾肯菌属等其他8个菌属各1种菌株。结论  安氏Ⅱ类错■畸形患者矫治前期龈沟液中分布多种细菌。

Abstract:

Objective To investigate the distribution of bacterial flora in gingival crevicular fluid of Angle Ⅱ malocclusion orthodontic patients in the early stage of correction, so as to figure out the influence of microbial infection on tooth root absorption in Angle Ⅱ malocclusion orthodontic patients after correction. Methods The gingival crevicular fluid of 24 Angle Ⅱ malocclusion orthodontic patients was collected from Xiangya Stomatological Hospital of Central South University. They were 12-18 years old. Then anaerobic cultivation was carried out. Bacterial 16S rRNA gene sequences were amplified by PCR, and then the bacteria strains were identified by sequence alignment. Results Forty-three strains of bacteria were identified, which included 6 strains of Actinomycetes, 18 strains of Streptococcus, 7 strains of Neisseria, 2 strains of Gemella, and 2 strains of Rothia, 1 strains of Eikenella and other 7 bactaria species. Conclusions There is a wide bacterial spectrum in gingival crevicular fluid of Angle Ⅱ malocclusion orthodontic patients in the early stage of correction.